PMID- 21104310
OWN - NLM
STAT- MEDLINE
DCOM- 20110502
LR  - 20211020
IS  - 1573-6849 (Electronic)
IS  - 0967-3849 (Linking)
VI  - 18
IP  - 7
DP  - 2010 Nov
TI  - Synteny between Brachypodium distachyon and Hordeum vulgare as revealed by FISH.
PG  - 841-50
LID - 10.1007/s10577-010-9166-3 [doi]
AB  - We investigated by fluorescence in situ hybridization (FISH) the synteny between 
      Brachypodium distachyon with a small genome (1C = 320 Mb) and barley with a large 
      genome (1C = 5,100 Mb) at the chromosome level. Reciprocal genomic in situ 
      hybridization (GISH) between B. distachyon and barley labeled mainly 45S 
      ribosomal DNA loci, indicating that most high copy DNA is weakly conserved 
      between both grasses. Of 13 BAC clones with inserts from different B. distachyon 
      chromosomes, only two belonging to chromosome 1 yielded hybridization signals on 
      a barley metaphase chromosome (on 7HS and 7HL, respectively), confirming synteny 
      between both chromosomes. FISH experiments to characterize the synteny of 
      single-copy loci were performed. Two of four Brachypodium sylvaticum BACs 
      spanning a 223-kb interval homologous to the region of barley that harbors a 
      gibberellic-acid-insensitive semi-dwarfing gene, sdw3, hybridized specifically to 
      a central position of B. distachyon chromosome 1 short arm but not to the 
      homologous region of the barley genome. Repeat-free sequences PCR amplified from 
      four non-overlapping barley BACs linked to the core of Sdw3 region yielded 
      signals at distinct positions in the middle of barley chromosome arm 2HS. 
      Together, these results (1) confirmed the synteny between B. distachyon 
      chromosome 1 and barley chromosomes 2H and 7H at the cytological level, (2) 
      indicated mid-arm position for the Sdw3 locus genetically mapped at the 
      centromere of barley chromosome 2H, and (3) proved that the sdw3 core interval of 
      < 100 kb in B. distachyon corresponds to a megabase-sized syntenic region in 
      barley.
FAU - Ma, Lu
AU  - Ma L
AD  - Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466 
      Gatersleben, Germany.
FAU - Vu, Giang T H
AU  - Vu GT
FAU - Schubert, Veit
AU  - Schubert V
FAU - Watanabe, Koichi
AU  - Watanabe K
FAU - Stein, Nils
AU  - Stein N
FAU - Houben, Andreas
AU  - Houben A
FAU - Schubert, Ingo
AU  - Schubert I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101123
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
SB  - IM
MH  - Brachypodium/*genetics/ultrastructure
MH  - Chromosomes, Artificial, Bacterial
MH  - Chromosomes, Plant/ultrastructure
MH  - Genetic Loci
MH  - Hordeum/*genetics/ultrastructure
MH  - In Situ Hybridization, Fluorescence
MH  - *Synteny
EDAT- 2010/11/26 06:00
MHDA- 2011/05/03 06:00
CRDT- 2010/11/25 06:00
PHST- 2010/09/15 00:00 [received]
PHST- 2010/10/25 00:00 [accepted]
PHST- 2010/10/22 00:00 [revised]
PHST- 2010/11/25 06:00 [entrez]
PHST- 2010/11/26 06:00 [pubmed]
PHST- 2011/05/03 06:00 [medline]
AID - 10.1007/s10577-010-9166-3 [doi]
PST - ppublish
SO  - Chromosome Res. 2010 Nov;18(7):841-50. doi: 10.1007/s10577-010-9166-3. Epub 2010 
      Nov 23.