PMID- 21104493
OWN - NLM
STAT- MEDLINE
DCOM- 20110315
LR  - 20160526
IS  - 1532-4117 (Electronic)
IS  - 1093-4529 (Linking)
VI  - 46
IP  - 1
DP  - 2011
TI  - Assessing the activity and diversity of fumarate-fed denitrifying bacteria by 
      performing field single-well push-pull tests.
PG  - 33-41
LID - 10.1080/10934529.2011.526080 [doi]
AB  - In situ biological denitrification has been proposed as an important metabolic 
      activity in the remediation of nitrate-contaminated groundwater. In this study, 
      the effects of fumarate, an electron donor for biological denitrification, on the 
      in situ denitrifying activity were determined by using three types of single-well 
      push-pull tests; transport, biostimulation and activity tests. During the tests, 
      changes in microbial community composition were also investigated using 
      denaturing gradient gel electrophoresis (DGGE) of 16S rRNA genes. Transport test 
      demonstrated that non-reactive tracer and biologically reactive solutes behaved 
      similarly. A biostimulation test was conducted to stimulate the denitrifying 
      activities of native microorganisms, which were monitored by detecting the 
      simultaneous production of CO(2) and drastic degradations of both nitrate and 
      fumarate after the injection of fumarate as an electron donor and/or carbon 
      source, with nitrate as an electron acceptor. A phylogenetic analysis suggested 
      that the taxonomic affiliation of the dominant species before biostimulation was 
      gamma-Proteobacteria, including Acinetobacter species and Pseudomonas fluorescens, 
      while the dominant species after biostimulation were affiliated with 
      beta-Proteobacteria, cytophaga-Flavobacterium-Bacteroides and high G+C gram-positive 
      bacteria. These results suggest that the analyses of groundwater samples using a 
      combination of single well push pull tests with DGGE can be applied to 
      investigate the activity, diversity and composition shift of denitrifying 
      bacteria in a nitrate-contaminated aquifer.
FAU - Kim, Jin-Hoon
AU  - Kim JH
AD  - Sudo Kwong Landfill Site Management Co., Incheon, Korea.
FAU - Ha, Chul-Yoon
AU  - Ha CY
FAU - Oa, Seong-Wook
AU  - Oa SW
FAU - Lee, Jin-Woo
AU  - Lee JW
FAU - Park, Sun-Hwa
AU  - Park SH
FAU - Kwon, Soo-Youl
AU  - Kwon SY
FAU - Kim, Sungpyo
AU  - Kim S
FAU - Kim, Young
AU  - Kim Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Environ Sci Health A Tox Hazard Subst Environ Eng
JT  - Journal of environmental science and health. Part A, Toxic/hazardous substances & 
      environmental engineering
JID - 9812551
RN  - 0 (Fumarates)
RN  - 0 (Nitrates)
SB  - IM
MH  - Bacteria/*metabolism
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Fumarates/*metabolism
MH  - Nitrates/*metabolism
EDAT- 2010/11/26 06:00
MHDA- 2011/03/16 06:00
CRDT- 2010/11/25 06:00
PHST- 2010/11/25 06:00 [entrez]
PHST- 2010/11/26 06:00 [pubmed]
PHST- 2011/03/16 06:00 [medline]
AID - 929741256 [pii]
AID - 10.1080/10934529.2011.526080 [doi]
PST - ppublish
SO  - J Environ Sci Health A Tox Hazard Subst Environ Eng. 2011;46(1):33-41. doi: 
      10.1080/10934529.2011.526080.