PMID- 21105739 OWN - NLM STAT- MEDLINE DCOM- 20110210 LR - 20101214 IS - 1520-5851 (Electronic) IS - 0013-936X (Linking) VI - 44 IP - 24 DP - 2010 Dec 15 TI - Understanding short-chain fatty acids accumulation enhanced in waste activated sludge alkaline fermentation: kinetics and microbiology. PG - 9343-8 LID - 10.1021/es102878m [doi] AB - Most of the studies on sewage sludge treatment in literature were conducted for methane generation under acidic or near neutral pH conditions. It was reported in our previous studies that the accumulation of short-chain fatty acids (SCFAs), the preferred carbon source of biological wastewater nutrient removal, was significantly enhanced when sludge was fermented under alkaline conditions, but the optimal pH was temperature-dependent (pH 10 at ambient temperature, pH 9 at mesophilic, and pH 8 at thermophilic), and the maximal SCFAs yields were in the following order: thermophilic pH 8 > mesophilic pH 9 > ambient pH 10 > ambient uncontrolled pH. In this study the kinetic and microbiological features of waste activated sludge fermented in the range of pH 7-10 were investigated to understand the mechanism of remarkably high SCFAs accumulation under alkaline conditions. The developed sludge alkaline fermentation model could be applied to predicate the experimental data in either batch or semicontinuous sludge alkaline fermentation tests, and the relationships among alkaline pH, kinetic parameters, and SCFAs were discussed. Further analyses with fluorescence in situ hybridization (FISH) and PCR-based 16S rRNA gene clone library indicated that both the ratio of bacteria to archaea and the fraction of SCFAs producer accounting for bacteria were in the sequence of thermophilic pH 8 > mesophilic pH 9 > ambient pH 10 > ambient uncontrolled pH, which was in correspondence with the observed order of maximal SCFAs yields. FAU - Zhang, Peng AU - Zhang P AD - State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, Shanghai 200092, China. FAU - Chen, Yinguang AU - Chen Y FAU - Zhou, Qi AU - Zhou Q FAU - Zheng, Xiong AU - Zheng X FAU - Zhu, Xiaoyu AU - Zhu X FAU - Zhao, Yuxiao AU - Zhao Y LA - eng SI - GENBANK/GU454862 SI - GENBANK/GU454863 SI - GENBANK/GU454864 SI - GENBANK/GU454865 SI - GENBANK/GU454866 SI - GENBANK/GU454867 SI - GENBANK/GU454868 SI - GENBANK/GU454869 SI - GENBANK/GU454870 SI - GENBANK/GU454871 SI - GENBANK/GU454872 SI - GENBANK/GU454873 SI - GENBANK/GU454874 SI - GENBANK/GU454875 SI - GENBANK/GU454876 SI - GENBANK/GU454877 SI - GENBANK/GU454878 SI - GENBANK/GU454879 SI - GENBANK/GU454880 SI - GENBANK/GU454881 SI - GENBANK/GU454882 SI - GENBANK/GU454883 SI - GENBANK/GU454884 SI - GENBANK/GU454885 SI - GENBANK/GU454886 SI - GENBANK/GU454887 SI - GENBANK/GU454888 SI - GENBANK/GU454889 SI - GENBANK/GU454890 SI - GENBANK/GU454891 SI - GENBANK/GU454892 SI - GENBANK/GU454893 SI - GENBANK/GU454894 SI - GENBANK/GU454895 SI - GENBANK/GU454896 SI - GENBANK/GU454897 SI - GENBANK/GU454898 SI - GENBANK/GU454899 SI - 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GENBANK/GU455360 SI - GENBANK/GU455361 SI - GENBANK/GU455362 SI - GENBANK/GU455363 SI - GENBANK/GU455364 SI - GENBANK/GU455365 SI - GENBANK/GU455366 SI - GENBANK/GU455367 SI - GENBANK/GU455368 SI - GENBANK/GU455369 SI - GENBANK/GU455370 SI - GENBANK/GU455371 SI - GENBANK/GU455372 SI - GENBANK/GU455373 SI - GENBANK/GU455374 SI - GENBANK/GU455375 SI - GENBANK/GU455376 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20101124 PL - United States TA - Environ Sci Technol JT - Environmental science & technology JID - 0213155 RN - 0 (Fatty Acids, Volatile) RN - 0 (Sewage) SB - IM MH - Anaerobiosis MH - Bacteria, Anaerobic/genetics/growth & development/metabolism MH - Base Sequence MH - Bioreactors/microbiology MH - Fatty Acids, Volatile/*analysis/metabolism MH - *Fermentation MH - Hydrogen-Ion Concentration MH - Hydrolysis MH - Kinetics MH - Molecular Sequence Data MH - Sewage/*chemistry/microbiology MH - Waste Disposal, Fluid/*methods EDAT- 2010/11/26 06:00 MHDA- 2011/02/11 06:00 CRDT- 2010/11/26 06:00 PHST- 2010/11/26 06:00 [entrez] PHST- 2010/11/26 06:00 [pubmed] PHST- 2011/02/11 06:00 [medline] AID - 10.1021/es102878m [doi] PST - ppublish SO - Environ Sci Technol. 2010 Dec 15;44(24):9343-8. doi: 10.1021/es102878m. Epub 2010 Nov 24.