PMID- 21107464
OWN - NLM
STAT- MEDLINE
DCOM- 20110506
LR  - 20191210
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 136
IP  - 3
DP  - 2011 Feb 7
TI  - Two-dimensional fingerprinting approach for comparison of complex substances 
      analysed by HPLC-UV and fluorescence detection.
PG  - 550-9
LID - 10.1039/c0an00639d [doi]
AB  - This work is concerned with the research and development of methodology for 
      analysis of complex mixtures such as pharmaceutical or food samples, which 
      contain many analytes. Variously treated samples (swill washed, fried and 
      scorched) of the Rhizoma atractylodis macrocephalae (RAM) traditional Chinese 
      medicine (TCM) as well as the common substitute, Rhizoma atractylodis (RA) TCM 
      were chosen as examples for analysis. A combined data matrix of chromatographic 
      2-D HPLC-DAD-FLD (two-dimensional high performance liquid chromatography with 
      diode array and fluorescence detectors) fingerprint profiles was constructed with 
      the use of the HPLC-DAD and HPLC-FLD individual data matrices; the purpose was to 
      collect maximum information and to interpret this complex data with the use of 
      various chemometrics methods e.g. the rank-ordering multi-criteria decision 
      making (MCDM) PROMETHEE and GAIA, K-nearest neighbours (KNN), partial least 
      squares (PLS), back propagation-artificial neural networks (BP-ANN) methods. The 
      chemometrics analysis demonstrated that the combined 2-D HPLC-DAD-FLD data matrix 
      does indeed provide more information and facilitates better performing 
      classification/prediction models for the analysis of such complex samples as the 
      RAM and RA ones noted above. It is suggested that this fingerprint approach is 
      suitable for analysis of other complex, multi-analyte substances.
FAU - Ni, Yongnian
AU  - Ni Y
AD  - Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 
      330047, China. ynni@ncu.edu.cn
FAU - Liu, Ying
AU  - Liu Y
FAU - Kokot, Serge
AU  - Kokot S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101125
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Complex Mixtures)
RN  - 0 (Drugs, Chinese Herbal)
SB  - IM
MH  - Atractylodes/chemistry
MH  - Calibration
MH  - Chromatography, High Pressure Liquid/instrumentation/*methods
MH  - Complex Mixtures/*analysis/standards
MH  - Drugs, Chinese Herbal/*analysis/standards
MH  - Least-Squares Analysis
MH  - Models, Theoretical
MH  - Neural Networks, Computer
MH  - Principal Component Analysis
MH  - Rhizome/chemistry
MH  - Spectrometry, Fluorescence
MH  - Spectrophotometry, Ultraviolet
EDAT- 2010/11/26 06:00
MHDA- 2011/05/07 06:00
CRDT- 2010/11/26 06:00
PHST- 2010/11/26 06:00 [entrez]
PHST- 2010/11/26 06:00 [pubmed]
PHST- 2011/05/07 06:00 [medline]
AID - 10.1039/c0an00639d [doi]
PST - ppublish
SO  - Analyst. 2011 Feb 7;136(3):550-9. doi: 10.1039/c0an00639d. Epub 2010 Nov 25.