PMID- 21108335
OWN - NLM
STAT- MEDLINE
DCOM- 20110302
LR  - 20101125
IS  - 1096-9071 (Electronic)
IS  - 0146-6615 (Linking)
VI  - 83
IP  - 1
DP  - 2011 Jan
TI  - In vitro study on vertical transmission of the HIV-1 gag gene by human sperm.
PG  - 16-23
LID - 10.1002/jmv.21767 [doi]
AB  - HIV/AIDS is a major public health problem worldwide. To explore the feasibility 
      of HIV vertical transmission by human sperm, plasmid construction and 
      transfection, interspecific in vitro fertilization of zona-free hamster ova by 
      human sperm, fluorescence in situ hybridization (FISH), RT-PCR, and 
      immunofluorescence assay (IFA) were carried out. The FISH signals for HIV-1 gag 
      DNA were observed in the nuclei and chromosomes of transfected human sperm, male 
      pronuclei of zygotes, and nuclei of blastomeres of two-cell embryos, indicating 
      that the HIV-1 gag gene could be transmitted via the sperm membrane and 
      integrated into the sperm genome. In contrast, human sperm carrying the target 
      gene achieved normal fertilization, and replication of the sperm-mediated target 
      gene was synchronized with the host genome. Using RT-PCR, the positive bands for 
      the target gene were observed in the transfected human sperm and two-cell 
      embryos. These results further confirm that the target gene can be transcribed 
      into mRNA in human sperm and embryonic cells. Positive signals for the HIV-1 p24 
      gag protein were shown by IFA in two-cell embryos containing the sperm-mediated 
      target gene and not in the transfected human sperm, which indicated that the 
      sperm-mediated target gene could be translated to make HIV-1 p24 gag protein in 
      embryonic cells, but not in sperm cells. The results provide evidence for 
      possible vertical transmission of the HIV-1 gag gene to the embryo by fertilizing 
      sperm in vitro.
CI  - (c) 2010 Wiley-Liss, Inc.
FAU - Wang, Dian
AU  - Wang D
AD  - Research Center for Reproductive Medicine, Shantou University Medical College, 
      Shantou, China.
FAU - Kang, Xiang-Jin
AU  - Kang XJ
FAU - Li, Lian-Bing
AU  - Li LB
FAU - Xie, Qing-Dong
AU  - Xie QD
FAU - Gao, Yu-Sen
AU  - Gao YS
FAU - Huang, Tian-Hua
AU  - Huang TH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (HIV Core Protein p24)
RN  - 0 (gag Gene Products, Human Immunodeficiency Virus)
SB  - IM
MH  - Animals
MH  - Cell Nucleus/virology
MH  - Cricetinae
MH  - Female
MH  - Fluorescent Antibody Technique, Direct
MH  - HIV Core Protein p24/biosynthesis
MH  - HIV Infections/*transmission/virology
MH  - HIV-1/genetics/*isolation & purification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Infectious Disease Transmission, Vertical
MH  - Male
MH  - Ovum/*virology
MH  - Protein Biosynthesis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spermatozoa/*virology
MH  - Transcription, Genetic
MH  - gag Gene Products, Human Immunodeficiency Virus/*genetics
EDAT- 2010/11/26 06:00
MHDA- 2011/03/03 06:00
CRDT- 2010/11/26 06:00
PHST- 2010/11/26 06:00 [entrez]
PHST- 2010/11/26 06:00 [pubmed]
PHST- 2011/03/03 06:00 [medline]
AID - 10.1002/jmv.21767 [doi]
PST - ppublish
SO  - J Med Virol. 2011 Jan;83(1):16-23. doi: 10.1002/jmv.21767.