PMID- 21108693
OWN - NLM
STAT- MEDLINE
DCOM- 20110324
LR  - 20110719
IS  - 1740-0929 (Electronic)
IS  - 1344-3941 (Linking)
VI  - 81
IP  - 6
DP  - 2010 Dec
TI  - Inhibitory effect of heat-killed Lactobacillus strain on immunoglobulin 
      E-mediated degranulation and late-phase immune reactions of mouse bone 
      marrow-derived mast cells.
PG  - 714-21
LID - 10.1111/j.1740-0929.2010.00788.x [doi]
AB  - This study investigated the in vitro effect of Lactobacillus strains, a major 
      group of probiotic lactic acid bacteria, on immunoglobulin E (IgE)- and 
      antigen-induced mast cell degranulation and subsequent gene expression. Bone 
      marrow-derived mast cells (BMMCs) from DBA/2 mice were cultured with heat-killed 
      Lactobacillus strains for 24 h. Some strains significantly inhibited IgE- and 
      antigen-induced beta-hexosaminidase release from BMMCs. Furthermore, Lactobacillus 
      reuteri NBRC 15892, which exhibited the strongest inhibitory activity, 
      significantly reduced the elevated interleukin (IL)-4, IL-13, tumor necrosis 
      factor-alpha, and cyclooxygenase-2 expression levels that was induced by 1-2 h of 
      stimulation with IgE and antigens. The suppressive effect of NBRC 15892 strain on 
      BMMC degranulation was significantly reduced in the presence of a toll-like 
      receptor (TLR)2-neutralizing antibody. In addition, downregulation of cell 
      surface FcepsilonRIalpha expression was observed after 6 h of NBRC 15892 treatment. These 
      results suggest that some Lactobacillus strains inhibited IgE-mediated mast cell 
      degranulation and subsequent late-phase reactions involving mast cells via a 
      TLR2-dependent mechanism with FcepsilonRIalpha downregulation.
CI  - (c) 2010 The Author; Journal compilation (c) 2010 Japanese Society of Animal Science.
FAU - Kawahara, Takeshi
AU  - Kawahara T
AD  - Shinshu University, Minamiminowa, Nagano, Japan. tkawafb@shinshu-u.ac.jp
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101026
PL  - Australia
TA  - Anim Sci J
JT  - Animal science journal = Nihon chikusan Gakkaiho
JID - 100956805
RN  - 0 (Interleukin-13)
RN  - 0 (Receptors, IgE)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 3.2.1.52 (beta-N-Acetylhexosaminidases)
SB  - IM
EIN - Anim Sci J. 2011 Jun;82(3):494
MH  - Animals
MH  - Bone Marrow Cells/*immunology/metabolism
MH  - *Cell Degranulation
MH  - Cyclooxygenase 2/metabolism
MH  - Down-Regulation
MH  - Immunoglobulin E/*immunology
MH  - Interleukin-13/metabolism
MH  - Interleukin-4/metabolism
MH  - *Lactobacillus
MH  - Male
MH  - Mast Cells/*immunology/metabolism
MH  - Mice
MH  - Mice, Inbred DBA
MH  - Probiotics/*pharmacology
MH  - Receptors, IgE/immunology
MH  - Toll-Like Receptor 2/immunology
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - beta-N-Acetylhexosaminidases/metabolism
EDAT- 2010/11/27 06:00
MHDA- 2011/03/25 06:00
CRDT- 2010/11/27 06:00
PHST- 2010/11/27 06:00 [entrez]
PHST- 2010/11/27 06:00 [pubmed]
PHST- 2011/03/25 06:00 [medline]
AID - 10.1111/j.1740-0929.2010.00788.x [doi]
PST - ppublish
SO  - Anim Sci J. 2010 Dec;81(6):714-21. doi: 10.1111/j.1740-0929.2010.00788.x. Epub 
      2010 Oct 26.