PMID- 21109292
OWN - NLM
STAT- MEDLINE
DCOM- 20110131
LR  - 20181201
IS  - 1527-9995 (Electronic)
IS  - 0090-4295 (Linking)
VI  - 77
IP  - 1
DP  - 2011 Jan
TI  - Effect of Serenoa repens, lycopene, and selenium on proinflammatory phenotype 
      activation: an in vitro and in vivo comparison study.
PG  - 248.e9-16
LID - 10.1016/j.urology.2010.07.514 [doi]
AB  - OBJECTIVES: To investigate the antiinflammatory activity of Serenoa repens (SeR), 
      LY, and) on proinflammatory phenotype in rat peritoneal macrophages (Ms) 
      stimulated with Salmonella enteritidis lipopolysaccharide (LPS) and in the 
      prostate of rats with partial bladder outlet obstruction. SeR, combined with 
      other compounds, such as LY and Se is used to relieve symptoms associated with 
      benign prostatic hyperplasia (BPH). Inflammation plays a pivotal role in the 
      pathogenesis of BPH and represents a target for anti-BPH drugs. METHODS: After 
      stimulation with 1 mug/mL of LPS, peritoneal rat MPhis were coincubated with LY (2 
      mug/mL), Se (0.03 mug/mL), and SeR (10 mug/mL), alone or in association (LY-Se-SeR) 
      and with RPMI. Inducible cyclooxygenase (COX-2), 5-lypoxygenase (5-LOX), 
      inducible nitric oxide synthase (iNOS), and inhibitor kappaBalpha (IkappaB-alpha) protein were 
      evaluated by Western blot. Nuclear factor-kappa B (NF-kappaB) binding activity was 
      measured by electrophoretic mobility shift assay. Tumor necrosis factor-alpha (TNF-alpha) 
      gene expression was investigated by real-time polymerase chain reaction. We also 
      evaluated malondialdehyde (MDA) and nitrite levels. RESULTS: LPS stimulation 
      produced a proinflammatory phenotype in rat peritoneal MPhis. LY, Se, and SeR 
      inhibited the inflammatory cascade, but the Ly-Se-SeR association caused a 
      greater inhibitory effect on the expression of COX-2, 5-LOX, and iNOS. The 
      Ly-Se-SeR association showed a higher efficacy in reducing the loss of IkappaB-alpha, the 
      increased NF-kappaB binding activity, the enhanced mRNA levels of TNF-alpha, the elevated 
      MDA, and nitrite content. The LY-Se-SeR association in vivo caused a greater 
      inhibitory effect on prostate inflammation induced in rats by partial bladder 
      outlet obstruction. CONCLUSIONS: The LY-Se-SeR association might be useful in the 
      treatment of BPH.
CI  - Copyright A(c) 2011 Elsevier Inc. All rights reserved.
FAU - Bonvissuto, Giulio
AU  - Bonvissuto G
AD  - Department of Urology, Policlinic Hospital, University of Catania, Italy.
FAU - Minutoli, Letteria
AU  - Minutoli L
FAU - Morgia, Giuseppe
AU  - Morgia G
FAU - Bitto, Alessandra
AU  - Bitto A
FAU - Polito, Francesca
AU  - Polito F
FAU - Irrera, Natasha
AU  - Irrera N
FAU - Marini, Herbert
AU  - Marini H
FAU - Squadrito, Francesco
AU  - Squadrito F
FAU - Altavilla, Domenica
AU  - Altavilla D
LA  - eng
PT  - Journal Article
DEP - 20101215
PL  - United States
TA  - Urology
JT  - Urology
JID - 0366151
RN  - 0 (Antioxidants)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (Nfkbia protein, rat)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
RN  - 36-88-4 (Carotenoids)
RN  - H6241UJ22B (Selenium)
RN  - SB0N2N0WV6 (Lycopene)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Carotenoids/*pharmacology
MH  - Cells, Cultured
MH  - I-kappa B Proteins/*drug effects/*genetics
MH  - Inflammation/immunology
MH  - Lycopene
MH  - Macrophages, Peritoneal/*drug effects
MH  - Male
MH  - NF-KappaB Inhibitor alpha
MH  - Phenotype
MH  - Prostate/drug effects/immunology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Selenium/*pharmacology
MH  - *Serenoa
MH  - Urinary Bladder Neck Obstruction/immunology
EDAT- 2010/11/27 06:00
MHDA- 2011/02/01 06:00
CRDT- 2010/11/27 06:00
PHST- 2010/02/16 00:00 [received]
PHST- 2010/07/21 00:00 [revised]
PHST- 2010/07/31 00:00 [accepted]
PHST- 2010/11/27 06:00 [entrez]
PHST- 2010/11/27 06:00 [pubmed]
PHST- 2011/02/01 06:00 [medline]
AID - S0090-4295(10)01540-2 [pii]
AID - 10.1016/j.urology.2010.07.514 [doi]
PST - ppublish
SO  - Urology. 2011 Jan;77(1):248.e9-16. doi: 10.1016/j.urology.2010.07.514. Epub 2010 
      Dec 15.