PMID- 21109600
OWN - NLM
STAT- MEDLINE
DCOM- 20110607
LR  - 20130926
IS  - 1522-1601 (Electronic)
IS  - 0161-7567 (Linking)
VI  - 110
IP  - 2
DP  - 2011 Feb
TI  - Electric field stimulation of precision-cut lung slices.
PG  - 545-54
LID - 10.1152/japplphysiol.00409.2010 [doi]
AB  - The precision-cut lung slice (PCLS) technique is widely used to examine airway 
      responses in different species. We developed a method to study nerve-dependent 
      bronchoconstriction by the application of electric field stimulation (EFS) to 
      PCLS. PCLS prepared from Wistar rats were placed between two platinum electrodes 
      to apply serial rectangular impulses (5-100 Hz), and bronchoconstriction was 
      studied by videomicroscopy. The extent of airway contractions increased with 
      higher frequencies. Stable repeated airway contractions were obtained at a 
      frequency of 50 Hz, a width of 1 ms, and an output of 200 mA for 2.5 s each 
      minute. Larger airways showed stronger responses. The EFS-triggered contractions 
      were increased by the acetylcholine esterase inhibitor neostigmine (10 muM) and 
      reversed by the muscarinic antagonist atropine (10 muM), whereas the thromboxane 
      protanoid receptor antagonist SQ29548 (10 muM) had no effect. Magnesium ions (10 
      mM) antagonized airway contractions induced by EFS, but not by methacholine, 
      indicating that nerve endings remain intact in PCLS. Our data further show that 
      the electrically evoked airway contractions in PCLS are mediated by cholinergic 
      nerves, independent of thromboxane and more prominent in larger airways. Taken 
      together these findings show that nerve endings remain intact in PCLS, and they 
      suggest that the present method is useful to study neurogenic responses in 
      airways of different size.
FAU - Schleputz, Marco
AU  - Schleputz M
AD  - Institute of Pharmacology and Toxicology, Univ. Hospital Aachen, Wendlingweg 2, 
      52074 Aachen. chmartin@ukaachen.de
FAU - Uhlig, Stefan
AU  - Uhlig S
FAU - Martin, Christian
AU  - Martin C
LA  - eng
PT  - Journal Article
DEP - 20101125
PL  - United States
TA  - J Appl Physiol (1985)
JT  - Journal of applied physiology (Bethesda, Md. : 1985)
JID - 8502536
SB  - IM
MH  - Animals
MH  - Bronchoconstriction/*physiology/radiation effects
MH  - Electric Stimulation/*methods
MH  - Female
MH  - Lung/*innervation/*physiology/radiation effects
MH  - Muscle Contraction/*physiology/radiation effects
MH  - Muscle, Smooth/innervation/*physiology/radiation effects
MH  - Organ Culture Techniques/*methods
MH  - Rats
MH  - Rats, Wistar
EDAT- 2010/11/27 06:00
MHDA- 2011/06/08 06:00
CRDT- 2010/11/27 06:00
PHST- 2010/11/27 06:00 [entrez]
PHST- 2010/11/27 06:00 [pubmed]
PHST- 2011/06/08 06:00 [medline]
AID - japplphysiol.00409.2010 [pii]
AID - 10.1152/japplphysiol.00409.2010 [doi]
PST - ppublish
SO  - J Appl Physiol (1985). 2011 Feb;110(2):545-54. doi: 
      10.1152/japplphysiol.00409.2010. Epub 2010 Nov 25.