PMID- 21111148
OWN - NLM
STAT- MEDLINE
DCOM- 20110411
LR  - 20131121
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 83
IP  - 2
DP  - 2010 Dec 15
TI  - Optimization of a novel method based on solidification of floating organic 
      droplet by high-performance liquid chromatography for evaluation of antifungal 
      drugs in biological samples.
PG  - 370-8
LID - 10.1016/j.talanta.2010.09.031 [doi]
AB  - In this study, a simple, rapid, and highly efficient liquid-phase microextraction 
      method based on solidification of floating organic droplet was coupled with high 
      performance liquid chromatography-photo diode array detection (HPLC-PDA) for 
      determination of ketoconazole, clotrimazole, and miconazole as antifungal drugs. 
      Central composite design (CCD) was used for optimization of several factors 
      affecting the extraction efficiency. The optimized conditions were established to 
      be 550 rpm for stirring rate, 35 min for extraction time, 57  degrees C for extraction 
      temperature, 8.5 for solution pH, 10 mul for organic solvent volume, and 7% (w/v) 
      of NaCl for ionic strength. Limit of detections (LODs) of the extraction method 
      ranged from 0.01 to 0.1 mug L(-1) and the linear dynamic ranges (LDRs) ranged from 
      0.1 to 300 mug L(-1) for the three antifungal drugs. Relative standard deviations 
      (RSDs) of the proposed method were 5-11%. Preconcentration factors in the range 
      of 306-1350 were obtained at extraction time of 35 min. Finally, performance of 
      the proposed method was evaluated for the extraction and determination of the 
      drugs' levels in microgram per liter in samples and satisfactory results were 
      obtained.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Adlnasab, Laleh
AU  - Adlnasab L
AD  - Department of Chemistry, Shahid Beheshti University, G.C., Evin, Tehran, Iran.
FAU - Ebrahimzadeh, Homeira
AU  - Ebrahimzadeh H
FAU - Yamini, Yadollah
AU  - Yamini Y
FAU - Mirzajani, Fateme
AU  - Mirzajani F
LA  - eng
PT  - Journal Article
DEP - 20101001
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Antifungal Agents)
RN  - 0 (Organic Chemicals)
RN  - 0 (Solvents)
RN  - 0 (Water Pollutants, Chemical)
RN  - 7NNO0D7S5M (Miconazole)
RN  - G07GZ97H65 (Clotrimazole)
RN  - R9400W927I (Ketoconazole)
SB  - IM
MH  - Antifungal Agents/*analysis
MH  - Chemistry Techniques, Analytical
MH  - Chemistry, Pharmaceutical/methods
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Clotrimazole/analysis
MH  - Ketoconazole/analysis
MH  - Light
MH  - Miconazole/analysis
MH  - Models, Chemical
MH  - Organic Chemicals/chemistry
MH  - Solvents/chemistry
MH  - Surface Properties
MH  - Temperature
MH  - Time Factors
MH  - Water Pollutants, Chemical/analysis
EDAT- 2010/11/30 06:00
MHDA- 2011/04/13 06:00
CRDT- 2010/11/30 06:00
PHST- 2010/06/12 00:00 [received]
PHST- 2010/09/09 00:00 [revised]
PHST- 2010/09/22 00:00 [accepted]
PHST- 2010/11/30 06:00 [entrez]
PHST- 2010/11/30 06:00 [pubmed]
PHST- 2011/04/13 06:00 [medline]
AID - S0039-9140(10)00745-9 [pii]
AID - 10.1016/j.talanta.2010.09.031 [doi]
PST - ppublish
SO  - Talanta. 2010 Dec 15;83(2):370-8. doi: 10.1016/j.talanta.2010.09.031. Epub 2010 
      Oct 1.