PMID- 21111453
OWN - NLM
STAT- MEDLINE
DCOM- 20110329
LR  - 20171116
IS  - 1532-8392 (Electronic)
IS  - 0046-8177 (Linking)
VI  - 42
IP  - 3
DP  - 2011 Mar
TI  - Dendritic cells in muscle lesions of sarcoidosis.
PG  - 340-6
LID - 10.1016/j.humpath.2010.07.011 [doi]
AB  - Sarcoidosis is a chronic systemic granulomatous disorder of unknown etiology. The 
      precise mechanism by which granulomatous lesions form is still obscure. Dendritic 
      cells (DCs) are the most efficient antigen presenting cells; however, pathologic 
      investigations of dendritic cells in the affected lesions of sarcoidosis are 
      quite limited. We immunohistochemically examined the localization and phenotypes 
      of dendritic cells and the expressions of CD40 and CD40L (CD154), which are key 
      molecules in dendritic cell activation, in the muscles of 5 patients with 
      muscular sarcoidosis, 8 patients with muscular disorders without inflammation, 
      and 4 patients with histologically normal muscles as controls. In muscular 
      sarcoidosis, CD1c-positive myeloid dendritic cells were scattered mainly in the 
      lymphocyte layers of granulomas and the endomysium around the granulomas. Double 
      immunostaining revealed that some CD1c-positive cells expressed the mature 
      dendritic cell marker CD83, but immature dendritic cell marker CD1a-positive 
      cells were not found. Smaller numbers of Blood dendritic cell antigen 
      (BDCA)-2-positive plasmacytoid dendritic cells were found in the lymphocyte 
      layers of granulomas. In the controls, small numbers of CD1c-positive cells were 
      seen in the endomysium, whereas BDCA-2-positive cells were not observed except in 
      1 case. In muscular sarcoidosis, CD40 was expressed on mononuclear cells, on the 
      interstitium around the muscle fibers and granulomas, and on the endothelium of 
      vessels. CD40L was positive on mononuclear cells scattered within and around 
      granulomas in 3 of 5 patients. In the controls, CD40 was expressed on the 
      endothelium of the vessels and sparse mononuclear cells in the lesions of muscle 
      fiber necrosis, whereas CD40L was not seen in any. In muscular sarcoidosis, 
      recruitment of myeloid dendritic cells and less plasmacytoid dendritic cells and 
      up-regulation of the CD40/CD40L system in affected muscles suggest that myeloid 
      dendritic cells may be mainly involved in granulomatous inflammation through 
      antigen presentation in a Th1 immune milieu.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Tateyama, Maki
AU  - Tateyama M
AD  - Department of Neurology, Tohoku University School of Medicine, Sendai 980-8574, 
      Japan. mtateyama@em.neurol.med.tohoku.ac.jp
FAU - Fujihara, Kazuo
AU  - Fujihara K
FAU - Itoyama, Yasuto
AU  - Itoyama Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101215
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (Antigens, CD1)
RN  - 0 (CD1C protein, human)
RN  - 0 (CD40 Antigens)
RN  - 0 (CLEC4C protein, human)
RN  - 0 (Glycoproteins)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Immunologic)
RN  - 147205-72-9 (CD40 Ligand)
SB  - IM
MH  - Antigens, CD1/metabolism
MH  - CD40 Antigens/metabolism
MH  - CD40 Ligand/metabolism
MH  - Child
MH  - Child, Preschool
MH  - Dendritic Cells/immunology/metabolism/*pathology
MH  - Endothelium, Vascular/metabolism
MH  - Fluorescent Antibody Technique, Indirect
MH  - Glycoproteins/metabolism
MH  - Granuloma/immunology/metabolism/pathology
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Immunohistochemistry
MH  - Infant
MH  - Lectins, C-Type/metabolism
MH  - Leukocytes, Mononuclear/metabolism/pathology
MH  - Membrane Glycoproteins/metabolism
MH  - Muscle, Skeletal/immunology/metabolism/*pathology
MH  - Receptors, Immunologic/metabolism
MH  - Sarcoidosis/immunology/metabolism/*pathology
EDAT- 2010/11/30 06:00
MHDA- 2011/03/30 06:00
CRDT- 2010/11/30 06:00
PHST- 2010/03/20 00:00 [received]
PHST- 2010/07/03 00:00 [revised]
PHST- 2010/07/08 00:00 [accepted]
PHST- 2010/11/30 06:00 [entrez]
PHST- 2010/11/30 06:00 [pubmed]
PHST- 2011/03/30 06:00 [medline]
AID - S0046-8177(10)00276-5 [pii]
AID - 10.1016/j.humpath.2010.07.011 [doi]
PST - ppublish
SO  - Hum Pathol. 2011 Mar;42(3):340-6. doi: 10.1016/j.humpath.2010.07.011. Epub 2010 
      Dec 15.