PMID- 21112833
OWN - NLM
STAT- MEDLINE
DCOM- 20110203
LR  - 20151119
IS  - 0236-5383 (Print)
IS  - 0236-5383 (Linking)
VI  - 61
IP  - 4
DP  - 2010 Dec
TI  - Effect of fungicide Euparen Multi (Tolylfluanid) on the induction of chromosomal 
      aberations in cultivated bovine lymphocytes.
PG  - 411-22
LID - 10.1556/ABiol.61.2010.4.5 [doi]
AB  - The effect of the fungicide Euparen Multi (containing 50% tolylfluanid) was 
      investigated on the induction of chromosomal aberrations (CA) in cultured bovine 
      peripheral lymphocytes. Cultures from two healthy donors were treated with 
      tolylfluanid-based fungicide at concentrations ranging from 1.7 to 17.5 mug/ml for 
      the last 24 and 48 hours of cultivation. Conventional cytogenetic method (CA 
      assay) with Giemsa staining as well as fluorescence in situ hybridization (FISH) 
      with whole bovine chromosomes 1 and 5 painting probes were used in the 
      experiment. In the CA assay, no clastogenic effect of the fungicide was found 
      after Euparen Multi treatment for 24 hours. On the contrary, significant 
      elevation in polyploidy induction was observed with dose-dependence in one of the 
      donors. Using prolonged time of exposure to the fungicide (the last 48 h of the 
      cultivation), a slight clastogenic effect was detected at the doses of 8.75 and 
      17.5 mug/ml (P < 0.05, P < 0.01, respectively) in donor 1 and at the dose of 8.75 
      mug/ml (P < 0.05) in donor 2. The highest doses tested caused reduction of the 
      mitotic indices (MI) (P < 0.05, P < 0.01) in both donors as well as both 
      treatment times. The evaluation of stable structural aberrations in lymphocytes 
      by two-colour FISH (48 h exposure) using bovine chromosome painting probes 
      revealed the presence of nonreciprocal translocations at two examined 
      concentrations (3.5 mug/ml and 8.75 mug/ml).
FAU - Orosova, Martina
AU  - Orosova M
AD  - Slovak Academy of Sciences, Parasitological Institute, Hlinkova, Kosice, Slovak 
      Republic.
FAU - Holeckova, Beata
AU  - Holeckova B
FAU - Sivikova, Katarina
AU  - Sivikova K
FAU - Dianovsky, J
AU  - Dianovsky J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Hungary
TA  - Acta Biol Hung
JT  - Acta biologica Hungarica
JID - 8404358
RN  - 0 (Aniline Compounds)
RN  - 0 (Antifungal Agents)
RN  - 0 (Azure Stains)
RN  - 76A92XU36Y (dichlofluanid)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Aniline Compounds/*pharmacology
MH  - Animals
MH  - Antifungal Agents/*pharmacology
MH  - Azure Stains/pharmacology
MH  - Cattle
MH  - Chromosome Aberrations/*drug effects
MH  - Chromosome Painting
MH  - Chromosomes/*drug effects
MH  - DNA/drug effects
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/*drug effects/metabolism
MH  - Mitotic Index
MH  - Polyploidy
MH  - Time Factors
MH  - Translocation, Genetic
EDAT- 2010/11/30 06:00
MHDA- 2011/02/04 06:00
CRDT- 2010/11/30 06:00
PHST- 2010/11/30 06:00 [entrez]
PHST- 2010/11/30 06:00 [pubmed]
PHST- 2011/02/04 06:00 [medline]
AID - 2367222L1234H303 [pii]
AID - 10.1556/ABiol.61.2010.4.5 [doi]
PST - ppublish
SO  - Acta Biol Hung. 2010 Dec;61(4):411-22. doi: 10.1556/ABiol.61.2010.4.5.