PMID- 21113291
OWN - NLM
STAT- MEDLINE
DCOM- 20110118
LR  - 20211020
IS  - 1472-3654 (Electronic)
IS  - 1472-3646 (Print)
IS  - 1472-3646 (Linking)
VI  - 2010
DP  - 2010 Nov 11
TI  - Two major archaeal pseudomurein endoisopeptidases: PeiW and PeiP.
PG  - 480492
LID - 10.1155/2010/480492 [doi]
LID - 480492
AB  - PeiW (UniProtKB Q7LYX0) and PeiP (UniProtKB Q77WJ4) are the two major 
      pseudomurein endoisopeptidases (Pei) that are known to cleave pseudomurein 
      cell-wall sacculi of the members of the methanogenic orders Methanobacteriales 
      and Methanopyrales. Both enzymes, originating from prophages specific for some 
      methanogenic archaeal species, hydrolyze the ϵ(Ala)-Lys bond of the peptide 
      linker between adjacent pseudomurein layers. Because lysozyme is not able to 
      cleave the pseudomurein cell wall, the enzymes are used in protoplast preparation 
      and in DNA isolation from pseudomurein cell-wall-containing methanogens. 
      Moreover, PeiW increases the probe permeability ratio and enables fluorescence in 
      situ hybridization (FISH) and catalyzed reporter deposition (CARD-) FISH 
      experiments to be performed on these methanogens.
FAU - Visweswaran, Ganesh Ram R
AU  - Visweswaran GR
AD  - Department of Molecular Genetics, GBB, University of Groningen, Kerklaan 30, 9751 
      NN Haren, The Netherlands.
FAU - Dijkstra, Bauke W
AU  - Dijkstra BW
FAU - Kok, Jan
AU  - Kok J
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20101111
PL  - United States
TA  - Archaea
JT  - Archaea (Vancouver, B.C.)
JID - 101142614
RN  - 0 (Peptidoglycan)
RN  - EC 3.4.- (Endopeptidases)
SB  - IM
MH  - Endopeptidases/*chemistry/genetics/*metabolism
MH  - Methanobacteriaceae/*enzymology
MH  - Peptidoglycan
PMC - PMC2989375
EDAT- 2010/11/30 06:00
MHDA- 2011/01/19 06:00
PMCR- 2010/11/11
CRDT- 2010/11/30 06:00
PHST- 2010/06/30 00:00 [received]
PHST- 2010/10/08 00:00 [accepted]
PHST- 2010/11/30 06:00 [entrez]
PHST- 2010/11/30 06:00 [pubmed]
PHST- 2011/01/19 06:00 [medline]
PHST- 2010/11/11 00:00 [pmc-release]
AID - 10.1155/2010/480492 [doi]
PST - epublish
SO  - Archaea. 2010 Nov 11;2010:480492. doi: 10.1155/2010/480492.