PMID- 21113640
OWN - NLM
STAT- MEDLINE
DCOM- 20110729
LR  - 20161125
IS  - 1439-7609 (Electronic)
IS  - 1439-7595 (Linking)
VI  - 21
IP  - 2
DP  - 2011 Apr
TI  - Increased level of heme oxygenase-1 in rheumatoid arthritis synovial fluid.
PG  - 150-7
LID - 10.1007/s10165-010-0372-9 [doi]
AB  - We investigated the expression and localization of heme oxygenase-1 (HO-1) in 
      synovial fluid and synovial tissue, and examined the stimulation of HO-1 
      production in rheumatoid synovial fibroblasts (RASFs). Synovial fluid samples 
      were obtained from knee joints of 20 rheumatoid arthritis (RA) and 20 
      osteoarthritis (OA) patients, and concentration of HO-1 and matrix 
      metalloproteinase-3 (MMP-3) were measured by enzyme-linked immunosorbent assay 
      (ELISA). Synovial tissues obtained from RA or OA patients during total knee 
      arthroplasty (TKA) were used for immunohistochemical analysis of HO-1. HO-1 
      production by RASFs in response to various cytokines was examined by ELISA. HO-1 
      levels in synovial fluid were higher in the RA group than in the OA group with 
      significant difference (P < 0.001), and correlated with serum C-reactive protein 
      (CRP) level (r = 0.80, P < 0.01) in the RA group. Higher levels of HO-1 were seen 
      in the RA-L group (Larsen grade III-V) than in the RA-E (Larsen grade 0-II) group 
      (P < 0.001). There was weak correlation between the levels of HO-1 protein and 
      MMP-3 in synovial fluid in the RA group (r = 0.31, P < 0.01), while no positive 
      correlation was observed in OA. Positive immunoreaction for HO-1 was observed in 
      cells of synovial tissue including synovial fibroblasts and cells in synovial 
      pannus. HO-1 protein levels in cultured media of RASFs were increased by 
      stimulation by interleukin-1beta at 6 h and tumor necrosis factor-alpha at 12 h, but 
      suppressed by interferon-gamma at 12 and 24 h. These results indicated that HO-1 
      expression in synovial tissue might be stimulated by inflammatory cytokines. The 
      correlation of HO-1 concentration in synovial fluid with serum CRP and MMP-3 in 
      joint fluid indicated that HO-1 might be useful as a marker of joint inflammation 
      in RA patients.
FAU - Kitamura, Ai
AU  - Kitamura A
AD  - Science of Functional Recovery and Reconstruction, Okayama University Graduate 
      School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
FAU - Nishida, Keiichiro
AU  - Nishida K
FAU - Komiyama, Takamitsu
AU  - Komiyama T
FAU - Doi, Hideyuki
AU  - Doi H
FAU - Kadota, Yasutaka
AU  - Kadota Y
FAU - Yoshida, Aki
AU  - Yoshida A
FAU - Ozaki, Toshifumi
AU  - Ozaki T
LA  - eng
PT  - Journal Article
DEP - 20101127
PL  - England
TA  - Mod Rheumatol
JT  - Modern rheumatology
JID - 100959226
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (Drug Combinations)
RN  - 0 (Lipopolysaccharides)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Arthritis, Rheumatoid/*enzymology/surgery
MH  - Arthroplasty, Replacement, Knee
MH  - Biomarkers/metabolism
MH  - Cytokines/pharmacology
MH  - Drug Combinations
MH  - Female
MH  - Fibroblasts/drug effects/metabolism
MH  - Heme Oxygenase-1/analysis/*metabolism
MH  - Humans
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Middle Aged
MH  - Osteoarthritis, Knee/enzymology/surgery
MH  - Synovial Fluid/chemistry/*enzymology
EDAT- 2010/11/30 06:00
MHDA- 2011/07/30 06:00
CRDT- 2010/11/30 06:00
PHST- 2010/01/28 00:00 [received]
PHST- 2010/10/13 00:00 [accepted]
PHST- 2010/11/30 06:00 [entrez]
PHST- 2010/11/30 06:00 [pubmed]
PHST- 2011/07/30 06:00 [medline]
AID - 10.1007/s10165-010-0372-9 [doi]
PST - ppublish
SO  - Mod Rheumatol. 2011 Apr;21(2):150-7. doi: 10.1007/s10165-010-0372-9. Epub 2010 
      Nov 27.