PMID- 21115244
OWN - NLM
STAT- MEDLINE
DCOM- 20110421
LR  - 20110110
IS  - 1873-2976 (Electronic)
IS  - 0960-8524 (Linking)
VI  - 102
IP  - 3
DP  - 2011 Feb
TI  - Evaluation of toxicity and degradation of a chlorophenol mixture by the laccase 
      produced by Trametes pubescens.
PG  - 3632-5
LID - 10.1016/j.biortech.2010.11.040 [doi]
AB  - In this study, the biodegradation of a mixture of 2-chlorophenol (2-CP), 
      2,4-dichlorophenol (2,4-DCP), 2,4,6-trichlorophenol (2,4,6-TCP) and 
      pentachlorophenol (PCP) using the laccase produced by the white-rot fungus 
      Trametes pubescens CBS 696.94 was evaluated. Two laccase isoenzymes with 
      molecular weights of about 60 and 120 kDa were identified in the enzymatic crude 
      extract. The highest laccase activity with syringaldazine was observed with pH 
      6.0 and 60 degrees C, while with 2,2-azino-bis(3-ethylbenzothiazoline-6) sulphonic acid 
      the highest activity was observed between 50 and 60 degrees C and 3.0-4.0 pH. A 
      biodegradation of 100%, 99%, 82.1% and 41.1% for 2-CP, 2,4-DCP, 2,4,6-TCP and 
      PCP, respectively, was observed after 4h of reaction. The reduction in 
      chlorophenols concentration allowed 90% reduction in mixture toxicity. In 
      summary, these results show the feasibility of a laccase enzymatic crude extract 
      from T. pubescens for the reduction of concentration and toxicity of 
      chlorophenols.
CI  - Copyright (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Gaitan, Ingrid J
AU  - Gaitan IJ
AD  - Chemical Engineering Department, Universidad de Los Andes, Bogota, Colombia.
FAU - Medina, Sandra C
AU  - Medina SC
FAU - Gonzalez, Juan C
AU  - Gonzalez JC
FAU - Rodriguez, Alexander
AU  - Rodriguez A
FAU - Espejo, Angela J
AU  - Espejo AJ
FAU - Osma, Johann F
AU  - Osma JF
FAU - Sarria, Victor
AU  - Sarria V
FAU - Almeciga-Diaz, Carlos J
AU  - Almeciga-Diaz CJ
FAU - Sanchez, Oscar F
AU  - Sanchez OF
LA  - eng
PT  - Journal Article
DEP - 20101113
PL  - England
TA  - Bioresour Technol
JT  - Bioresource technology
JID - 9889523
RN  - 0 (Chlorophenols)
RN  - EC 1.10.3.2 (Laccase)
SB  - IM
MH  - Biodegradation, Environmental
MH  - Cell Survival/*drug effects
MH  - Chlorophenols/*chemistry/*toxicity
MH  - Laccase/*chemistry/*metabolism
MH  - Trametes/*enzymology
EDAT- 2010/12/01 06:00
MHDA- 2011/04/22 06:00
CRDT- 2010/12/01 06:00
PHST- 2010/08/12 00:00 [received]
PHST- 2010/11/04 00:00 [revised]
PHST- 2010/11/09 00:00 [accepted]
PHST- 2010/12/01 06:00 [entrez]
PHST- 2010/12/01 06:00 [pubmed]
PHST- 2011/04/22 06:00 [medline]
AID - S0960-8524(10)01842-0 [pii]
AID - 10.1016/j.biortech.2010.11.040 [doi]
PST - ppublish
SO  - Bioresour Technol. 2011 Feb;102(3):3632-5. doi: 10.1016/j.biortech.2010.11.040. 
      Epub 2010 Nov 13.