PMID- 21116688
OWN - NLM
STAT- MEDLINE
DCOM- 20110620
LR  - 20211020
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 349
IP  - 1-2
DP  - 2011 Mar
TI  - Antioxidative and radioprotective activities of semiquinone glucoside derivative 
      (SQGD) isolated from Bacillus sp. INM-1.
PG  - 57-67
LID - 10.1007/s11010-010-0660-x [doi]
AB  - A semiquinone glucoside derivative (SQGD) was isolated from a radioresistant 
      bacterium Bacillus sp. INM-1 and its antioxidant and radioprotective activities 
      were evaluated using in vitro assays. Natural stable free radical properties of 
      SQGD in solid as well as in solution form were estimated using Electron 
      Paramagnetic Resonance (EPR) spectrometry. Results of the study were demonstrated 
      high reducing power (1.267 +/- 0.03356 U(abs)) and nitric oxide radicals scavenging 
      activity (34.684 +/- 2.132%) of SQGD. Maximum lipid peroxidation inhibitory 
      activity of SQGD was found to be 74.09 +/- 0.08% at 500 mug/ml concentration. 
      Similarly, significant (39.54%; P < 0.05) protection to the liposomal artificial 
      membrane against gamma radiation was observed by SQGD in terms of neutralization 
      of gamma radiation-induced TBARS radicals in vitro. OH(-) radicals scavenging 
      efficacy of SQGD was estimated in terms of % inhibition in deoxy D: -ribose 
      degradation by non-site-specific and site-specific assay. The maximum (54.01 +/- 
      1.01%) inhibition of deoxy D: -ribose degradation was observed in 
      non-site-specific manner, whereas, site-specific inhibition was observed to be 
      46.36 +/- 0.5% at the same concentration (250 mug/ml) of SQGD. EPR spectroscopic 
      analysis of the SQGD indicated ~80% reduction of DPPH radicals at 6.4% 
      concentration. EPR spectral analysis of SQGD was revealed an appearance of very 
      strong EPR signal of 2.00485 (crystalline form) and 2.00520 (solution form) g(y) 
      tensor value, which were an established characteristic of o-semiquinone radicals. 
      Therefore, it can be concluded that SQGD is a natural stable o-semiquinone-type 
      radical, possessing strong antioxidant activities and can effectively neutralize 
      radiation induced free radicals in biological system.
FAU - Kumar, Raj
AU  - Kumar R
AD  - Institute of Nuclear Medicine and Allied Sciences, Delhi, India. 
      rajkumar790@yahoo.com
FAU - Bansal, Deen Dayal
AU  - Bansal DD
FAU - Patel, Dev Dutt
AU  - Patel DD
FAU - Mishra, Saurabh
AU  - Mishra S
FAU - Karamalakova, Yana
AU  - Karamalakova Y
FAU - Zheleva, A
AU  - Zheleva A
FAU - Gadjeva, Vessilina
AU  - Gadjeva V
FAU - Sharma, Rakesh Kumar
AU  - Sharma RK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101130
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Antioxidants)
RN  - 0 (Benzothiazoles)
RN  - 0 (Biphenyl Compounds)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Glucosides)
RN  - 0 (Liposomes)
RN  - 0 (Picrates)
RN  - 0 (Radiation-Protective Agents)
RN  - 0 (Sulfonic Acids)
RN  - 28752-68-3 (2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 3352-57-6 (Hydroxyl Radical)
RN  - DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Antioxidants/chemistry/*isolation & purification
MH  - Ascorbic Acid/chemistry
MH  - Bacillus/*chemistry
MH  - Benzothiazoles/chemistry
MH  - Biphenyl Compounds/chemistry
MH  - Electron Spin Resonance Spectroscopy
MH  - Free Radical Scavengers/chemistry/*isolation & purification
MH  - Glucosides/chemistry/*isolation & purification
MH  - Hydroxyl Radical/chemistry
MH  - Lipid Peroxidation
MH  - Liposomes/chemistry/radiation effects
MH  - Nitric Oxide/chemistry
MH  - Oxidation-Reduction
MH  - Picrates/chemistry
MH  - Radiation-Protective Agents/chemistry/*isolation & purification
MH  - Sulfonic Acids/chemistry
EDAT- 2010/12/01 06:00
MHDA- 2011/06/21 06:00
CRDT- 2010/12/01 06:00
PHST- 2010/07/23 00:00 [received]
PHST- 2010/11/15 00:00 [accepted]
PHST- 2010/12/01 06:00 [entrez]
PHST- 2010/12/01 06:00 [pubmed]
PHST- 2011/06/21 06:00 [medline]
AID - 10.1007/s11010-010-0660-x [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2011 Mar;349(1-2):57-67. doi: 10.1007/s11010-010-0660-x. Epub 
      2010 Nov 30.