PMID- 21117028
OWN - NLM
STAT- MEDLINE
DCOM- 20110324
LR  - 20170213
IS  - 1699-5848 (Electronic)
IS  - 0213-3911 (Linking)
VI  - 26
IP  - 1
DP  - 2011 Jan
TI  - CDC28 protein kinase regulatory subunit 1B (CKS1B) expression and genetic status 
      analysis in oral squamous cell carcinoma.
PG  - 71-7
LID - 10.14670/HH-26.71 [doi]
AB  - CKS1B is a member of the highly conserved cyclin kinase subunit 1 (CKS1) protein 
      family which interacts with cyclin-dependent kinases and plays a critical role in 
      cell cycle progression. In oral squamous cell carcinoma (OSCC), as in other 
      malignancies, CKS1B overexpression has been correlated with reduced survival. To 
      our knowledge, no studies evaluating the genetic status of CKS1B gene in OSCC 
      have been reported. Herein, genetic and protein status of CKS1B were analyzed by 
      immunohistochemical (IHC) and fluorescence in situ hybridization (FISH) 
      techniques in a series of primary OSCC (n=51) and lymph node OSCC metastases 
      samples (n=14). The observed results were compared with those obtained in either 
      inflammatory (oral lichen planus [OLP]) (n=13) and premalignant oral mucosal 
      lesions (oral leukoplakia) (n=16). A significant CKS1B overexpression was 
      observed in OSCC and lymph node metastases samples than in OLP and oral 
      leukoplakia (mean 70% vs 35%, p<0.001). CKS1B overexpression correlated with p27 
      loss of expression (p=0.0013) and SKP2 overexpression (p<0.00). FISH study 
      disclosed statistical differences in both gene amplifications and gains between 
      samples corresponding to OSCC and metastases from those of OLP and leukoplakia 
      (p<0.001). Amplifications were present in 53% of OSCC samples and 33% of lymph 
      node metastases vs 14% of oral leukoplakia and 0% of OLP biopsy specimens 
      (p=0.002). Polysomies of chromosome 1 were seen in 46% of OSCC, 33% of ganglionar 
      metastases, 14% of oral leukoplakia and 10% of OLP (p=0.036). Correlation of 
      CKS1B over-expression and gains (both polysomies and amplifications) determined 
      by FISH was statistically significant (p<0.001). Our results indicate that a high 
      CKS1B expression is a common finding in primary OSCC which correlates with p27 
      low expression and SKP2 overexpression. This phenomenon may be due either to 
      numerical (chromosome 1 polysomy) or structural (amplifications) CKS1B genetic 
      abnormalities. This phenotypical and cytogenetic profile is not observed in 
      premalignant or inflammatory oral mucosal lesions.
FAU - Martin-Ezquerra, Gemma
AU  - Martin-Ezquerra G
AD  - Department of Medicine and Dermatology, Universitat Autonoma de Barcelona, 
      Hospital del Mar, Barcelona, Spain. GMartin@hospitaldelmar.cat
FAU - Salgado, Rocio
AU  - Salgado R
FAU - Toll, Agusti
AU  - Toll A
FAU - Baro, Teresa
AU  - Baro T
FAU - Mojal, Sergi
AU  - Mojal S
FAU - Yebenes, Mireia
AU  - Yebenes M
FAU - Garcia-Muret, Ma Pilar
AU  - Garcia-Muret MP
FAU - Sole, Francesc
AU  - Sole F
FAU - Quitllet, Francesc Alameda
AU  - Quitllet FA
FAU - Espinet, Blanca
AU  - Espinet B
FAU - Pujol, Ramon M
AU  - Pujol RM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Spain
TA  - Histol Histopathol
JT  - Histology and histopathology
JID - 8609357
RN  - 0 (CDKN1B protein, human)
RN  - 0 (CKS1B protein, human)
RN  - 0 (Carrier Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (S-Phase Kinase-Associated Proteins)
RN  - 147604-94-2 (Cyclin-Dependent Kinase Inhibitor p27)
RN  - EC 2.7.11.22 (CDC2-CDC28 Kinases)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - CDC2-CDC28 Kinases
MH  - Carcinoma, Squamous Cell/*enzymology/*genetics/pathology/secondary
MH  - Carrier Proteins/*genetics/*metabolism
MH  - Chromosome Aberrations
MH  - Cyclin-Dependent Kinase Inhibitor p27
MH  - Cyclin-Dependent Kinases/*genetics/*metabolism
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Intracellular Signaling Peptides and Proteins/genetics/metabolism
MH  - Leukoplakia, Oral/enzymology/genetics
MH  - Lichen Planus, Oral/enzymology/genetics
MH  - Lymphatic Metastasis/genetics
MH  - Male
MH  - Middle Aged
MH  - Mouth Neoplasms/*enzymology/*genetics/pathology
MH  - S-Phase Kinase-Associated Proteins/genetics/metabolism
EDAT- 2010/12/01 06:00
MHDA- 2011/03/25 06:00
CRDT- 2010/12/01 06:00
PHST- 2010/12/01 06:00 [entrez]
PHST- 2010/12/01 06:00 [pubmed]
PHST- 2011/03/25 06:00 [medline]
AID - 10.14670/HH-26.71 [doi]
PST - ppublish
SO  - Histol Histopathol. 2011 Jan;26(1):71-7. doi: 10.14670/HH-26.71.