PMID- 21117624
OWN - NLM
STAT- MEDLINE
DCOM- 20110324
LR  - 20161125
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 83
IP  - 1
DP  - 2011 Jan 1
TI  - Cation exchange in ZnSe nanocrystals for signal amplification in bioassays.
PG  - 402-8
LID - 10.1021/ac102688s [doi]
AB  - ZnSe nanocrystals (NCs), possessing low native luminescence but high 
      biocompatibility, were employed as labeling tags in bioassays. They were able to 
      amplify each target recognition event thousands of times through a 
      cation-exchange reaction (CXAmp) that released over 3000 encapsulated Zn(2+) from 
      one single NC. The freed cations in turn triggered strong fluorescence from the 
      Zn-responsive dyes. The present study demonstrated that CXAmp with ZnSe delivered 
      superior detection performance in comparison to the conventional labeling 
      methods. The overall fluorescence intensity of CXAmp using 5 nM ZnSe NCs was 30 
      times higher than that from 5 nM core-shell CdSe/ZnS quantum dots (QDs). The 
      limit of detection (LOD) obtained with ZnSe-based CXAmp was 10-fold lower than 
      with horseradish peroxidase (HRP) labeling, and the detection sensitivity, 
      represented by the slope of the signal-versus-concentration curve, was 20-fold 
      higher. When applied to detect immunoglobulin E (IgE) in a sandwich format, a LOD 
      of 1 ng/mL was achieved. The highly sensitive CXAmp also allowed detection of the 
      total IgE content in dilute human serum, in which the abundant matrix proteins 
      exhibited less interference and more accurate quantification could be performed. 
      Besides high signal amplification efficiency and good biocompatibility, CXAmp 
      with ZnSe could be easily adapted to common laboratory settings and act as a 
      universal labeling system for reliable detection of low-abundance targets.
FAU - Yao, Jingjing
AU  - Yao J
AD  - Department of Chemistry, University of California, Riverside, California 
      92521-0403, United States.
FAU - Schachermeyer, Samantha
AU  - Schachermeyer S
FAU - Yin, Yadong
AU  - Yin Y
FAU - Zhong, Wenwan
AU  - Zhong W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101130
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Selenium Compounds)
RN  - 0 (Zinc Compounds)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - OWX23150D5 (zinc selenide)
SB  - IM
MH  - Animals
MH  - Biological Assay/*methods
MH  - Humans
MH  - Immunoglobulin E/analysis
MH  - Ion Exchange
MH  - Nanoparticles/*chemistry
MH  - Selenium Compounds/*chemistry
MH  - Spectrometry, Fluorescence
MH  - Zinc Compounds/*chemistry
EDAT- 2010/12/02 06:00
MHDA- 2011/03/25 06:00
CRDT- 2010/12/02 06:00
PHST- 2010/12/02 06:00 [entrez]
PHST- 2010/12/02 06:00 [pubmed]
PHST- 2011/03/25 06:00 [medline]
AID - 10.1021/ac102688s [doi]
PST - ppublish
SO  - Anal Chem. 2011 Jan 1;83(1):402-8. doi: 10.1021/ac102688s. Epub 2010 Nov 30.