PMID- 21119931
OWN - NLM
STAT- MEDLINE
DCOM- 20110314
LR  - 20211020
IS  - 1943-4731 (Electronic)
IS  - 1524-0215 (Print)
IS  - 1524-0215 (Linking)
VI  - 21
IP  - 4
DP  - 2010 Dec
TI  - Evaluation of GC-APCI/MS and GC-FID as a complementary platform.
PG  - 205-13
AB  - With a development of the metabolomics field, complementary cross-platform 
      approaches started to attract attention, as none of the contemporary analytical 
      methods had the capacity to cover the entire space of the human metabolome. In 
      the current manuscript, we have evaluated an online coupling of gas 
      chromatography (GC)-mass spectrometry (MS) and flame ionization detector (FID) as 
      ways of cross-detector analysis. The possible value of this combination was 
      recognized from the very first days of GC-MS history but was never explored in 
      detail. We have compared the basic analytical parameters of both detectors, such 
      as limit of detection (LOD) and limit of quantification, with intra- and interday 
      reproducibility. We show that for the majority of the tested compounds, MS 
      detector demonstrates lower LOD. At the same time, FID appeared to be more 
      robust, showing lower relative standard deviations (RSDs) for intra- and interday 
      reproducibility. We conclude that the gain of this dual detector acquisition 
      appears to be most evident for complex biological samples, where wide dynamic 
      range and predictable response of FID are useful for an initial quantitative 
      overview of sample composition and estimation of molar proportions of different 
      metabolites. MS provides reliable, structural information and superior, at least 
      in the case of atmospheric pressure chemical ionization, sensitivity. Taken 
      together, both detectors represent a flexible tool for explorative studies and if 
      supported by a powerful data-processing algorithm, would appear to be useful in 
      any metabolic profiling study.
FAU - Pacchiarotta, Tiziana
AU  - Pacchiarotta T
AD  - Leiden University Medical Center, Biomolecular Mass Spectrometry Unit, Department 
      of Parasitology, Leiden, The Netherlands.
FAU - Nevedomskaya, Ekaterina
AU  - Nevedomskaya E
FAU - Carrasco-Pancorbo, Alegria
AU  - Carrasco-Pancorbo A
FAU - Deelder, Andre M
AU  - Deelder AM
FAU - Mayboroda, Oleg A
AU  - Mayboroda OA
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - J Biomol Tech
JT  - Journal of biomolecular techniques : JBT
JID - 100888641
SB  - IM
MH  - Atmospheric Pressure
MH  - Chromatography, Gas
MH  - *Flame Ionization
MH  - *Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Mass Spectrometry
MH  - Metabolome
MH  - Metabolomics/*methods
PMC - PMC2977964
OTO - NOTNLM
OT  - atmospheric pressure chemical ionization
OT  - gas chromatography
OT  - mass spectrometry
OT  - metabolomics
EDAT- 2010/12/02 06:00
MHDA- 2011/03/15 06:00
PMCR- 2010/12/01
CRDT- 2010/12/02 06:00
PHST- 2010/12/02 06:00 [entrez]
PHST- 2010/12/02 06:00 [pubmed]
PHST- 2011/03/15 06:00 [medline]
PHST- 2010/12/01 00:00 [pmc-release]
AID - 3644776 [pii]
PST - ppublish
SO  - J Biomol Tech. 2010 Dec;21(4):205-13.