PMID- 21120695
OWN - NLM
STAT- MEDLINE
DCOM- 20110809
LR  - 20211020
IS  - 1573-9686 (Electronic)
IS  - 0090-6964 (Print)
IS  - 0090-6964 (Linking)
VI  - 39
IP  - 4
DP  - 2011 Apr
TI  - Design of experiments approach to engineer cell-secreted matrices for directing 
      osteogenic differentiation.
PG  - 1174-85
LID - 10.1007/s10439-010-0217-x [doi]
AB  - The presentation of extracellular matrix (ECM) proteins provides an opportunity 
      to instruct the phenotype and behavior of responsive cells. Decellularized 
      cell-secreted matrix coatings (DM) represent a biomimetic culture surface that 
      retains the complexity of the natural ECM. Microenvironmental culture conditions 
      alter the composition of these matrices and ultimately the ability of DMs to 
      direct cell fate. We employed a design of experiments (DOE) multivariable 
      analysis approach to determine the effects and interactions of four variables 
      (culture duration, cell seeding density, oxygen tension, and media 
      supplementation) on the capacity of DMs to direct the osteogenic differentiation 
      of human mesenchymal stem cells (hMSCs). DOE analysis revealed that matrices 
      created with extended culture duration, ascorbate-2-phosphate supplementation, 
      and in ambient oxygen tension exhibited significant correlations with enhanced 
      hMSC differentiation. We validated the DOE model results using DMs predicted to 
      have superior (DM1) or lesser (DM2) osteogenic potential for naive hMSCs. 
      Compared to cells on DM2, hMSCs cultured on DM1 expressed 2-fold higher osterix 
      levels and deposited 3-fold more calcium over 3 weeks. Cells on DM1 coatings also 
      exhibited greater proliferation and viability compared to DM2-coated substrates. 
      This study demonstrates that DOE-based analysis is a powerful tool for optimizing 
      engineered systems by identifying significant variables that have the greatest 
      contribution to the target output.
FAU - Decaris, Martin L
AU  - Decaris ML
AD  - Department of Biomedical Engineering, University of California, 451 Health 
      Sciences Drive, Davis, CA 95616, USA.
FAU - Leach, J Kent
AU  - Leach JK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101201
PL  - United States
TA  - Ann Biomed Eng
JT  - Annals of biomedical engineering
JID - 0361512
RN  - 0 (Extracellular Matrix Proteins)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Biomedical Engineering
MH  - Biomimetic Materials
MH  - Calcium/metabolism
MH  - Cell Adhesion
MH  - Cell Differentiation/physiology
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Extracellular Matrix Proteins/physiology
MH  - Gene Expression Regulation, Developmental
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/*physiology
MH  - Models, Biological
MH  - Osteoblasts/cytology/physiology
MH  - Osteogenesis/genetics/*physiology
MH  - Polymerase Chain Reaction
MH  - Tissue Engineering
PMC - PMC3069311
EDAT- 2010/12/02 06:00
MHDA- 2011/08/10 06:00
PMCR- 2010/12/01
CRDT- 2010/12/02 06:00
PHST- 2010/08/31 00:00 [received]
PHST- 2010/11/19 00:00 [accepted]
PHST- 2010/12/02 06:00 [entrez]
PHST- 2010/12/02 06:00 [pubmed]
PHST- 2011/08/10 06:00 [medline]
PHST- 2010/12/01 00:00 [pmc-release]
AID - 217 [pii]
AID - 10.1007/s10439-010-0217-x [doi]
PST - ppublish
SO  - Ann Biomed Eng. 2011 Apr;39(4):1174-85. doi: 10.1007/s10439-010-0217-x. Epub 2010 
      Dec 1.