PMID- 21122484
OWN - NLM
STAT- MEDLINE
DCOM- 20110215
LR  - 20101202
IS  - 1635-7922 (Print)
IS  - 1635-7922 (Linking)
VI  - 9
IP  - 1
DP  - 2010 Jan-Mar
TI  - Bacterial and fungal communities in the rhizosphere of field-grown genetically 
      modified pine trees (Pinus radiata D.).
PG  - 25-40
LID - 10.1051/ebr/2010007 [doi]
AB  - This study assessed the impact of Pinus radiata (D. Don) genetically modified 
      (GM) by biolistic insertion of the LEAFY and nptII genes on rhizosphere microbial 
      communities of field grown trees. Rhizosphere soil was sampled quarterly for two 
      consecutive years. A culture-independent approach was used to characterise the 
      microbial communities based on PCR and denaturing gradient gel electrophoresis 
      (DGGE) of 16S/18S rDNA gene fragments, and internal transcribed spacer (ITS) 
      fragments amplified from total rhizosphere DNA. Trees from two independent 
      transformation events were sampled, together with non-modified control trees of 
      the same parental genotype. DGGE profiles of rhizosphere general Bacteria did not 
      differ between GM and control trees with one exception (summer 2006 sample). For 
      Alphaproteo- and Actinobacteria, significant differences between treatments were 
      detected in one out of eight samplings. Small seasonal shifts could be detected 
      in all bacterial communities. General fungal and ectomycorrhizal communities did 
      not differ significantly between GM and control trees with the exception of 
      summer 2006, when ectomycorrhizal communities associated with GM trees from one 
      transformation event differed from those associated with control trees. Small 
      seasonal shifts of general fungal and ectomycorrhizal communities were seen over 
      the two-year sampling period. More detailed analysis of microbial communities at 
      one sampling date (using amplified rDNA restriction analysis (ARDRA) and 16S/18S 
      rDNA sequencing) revealed significant differences in four ARDRA groups between 
      one GM treatment and the control (bacteria), and significant differences in one 
      ARDRA group between the two GM treatments (fungi). When data from all sampling 
      dates are considered together, the low incidence of statistical differences in 
      the microbial communities associated with the genetically modified and control 
      trees suggests that there was no significant impact of this genetic modification 
      on rhizosphere microbial communities.
CI  - (c) ISBR, EDP Sciences, 2010.
FAU - Lottmann, Jana
AU  - Lottmann J
AD  - AgResearch Limited, Biocontrol and Biosecurity Section, Christchurch, New 
      Zealand. jana.monk@agresearch.co.nz
FAU - O'Callaghan, Maureen
AU  - O'Callaghan M
FAU - Baird, David
AU  - Baird D
FAU - Walter, Christian
AU  - Walter C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101111
PL  - France
TA  - Environ Biosafety Res
JT  - Environmental biosafety research
JID - 101172622
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Fungal)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Fungal)
RN  - 0 (RNA, Ribosomal, 18S)
SB  - IM
MH  - Bacteria/*classification/isolation & purification
MH  - Cloning, Molecular
MH  - DNA, Bacterial/genetics
MH  - DNA, Fungal/genetics
MH  - Fungi/*classification/isolation & purification
MH  - Pinus/*genetics/*microbiology
MH  - Plant Roots/*microbiology
MH  - Plants, Genetically Modified/*microbiology
MH  - Polymerase Chain Reaction
MH  - RNA, Bacterial/genetics
MH  - RNA, Fungal/genetics
MH  - RNA, Ribosomal, 18S/genetics
MH  - Soil Microbiology
EDAT- 2010/12/03 06:00
MHDA- 2011/02/16 06:00
CRDT- 2010/12/03 06:00
PHST- 2009/10/14 00:00 [received]
PHST- 2010/08/20 00:00 [accepted]
PHST- 2010/12/03 06:00 [entrez]
PHST- 2010/12/03 06:00 [pubmed]
PHST- 2011/02/16 06:00 [medline]
AID - ebr1002 [pii]
AID - 10.1051/ebr/2010007 [doi]
PST - ppublish
SO  - Environ Biosafety Res. 2010 Jan-Mar;9(1):25-40. doi: 10.1051/ebr/2010007. Epub 
      2010 Nov 11.