PMID- 21123653
OWN - NLM
STAT- MEDLINE
DCOM- 20101221
LR  - 20240109
IS  - 1549-5477 (Electronic)
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 24
IP  - 23
DP  - 2010 Dec 1
TI  - MicroRNAs prevent precocious gene expression and enable pattern formation during 
      plant embryogenesis.
PG  - 2678-92
LID - 10.1101/gad.1986710 [doi]
AB  - Arabidopsis embryos lacking DICER-LIKE1 (DCL1), which is required for microRNA 
      (miRNA) biogenesis, arrest early in development. To assess the functions of 
      embryonic miRNAs, we determined the developmental and molecular consequences of 
      DCL1 loss. We found that DCL1 is required for cell differentiation events as 
      early as the eight-cell stage and soon thereafter for proper division of the 
      hypophysis and subprotoderm cells. By the early globular ( approximately 32-cell) stage, 
      dcl1-null mutant embryos overexpress  approximately 50 miRNA targets. In dcl1 eight-cell 
      embryos, the two most up-regulated targets are those of miR156 and encode SPL10 
      and SPL11 transcription factors. SPL10 and SPL11 are derepressed >150-fold in 
      dcl1 embryos and are redundantly required for the dcl1 early patterning defects. 
      Moreover, as early as the eight-cell stage, miR156-mediated repression of zygotic 
      SPL transcripts prevents premature accumulation of transcripts from genes 
      normally induced during the embryonic maturation phase. Thus, the first 
      perceptible molecular function of plant embryonic miRNAs is the opposite of that 
      in vertebrates; in vertebrates, miRNAs sharpen the first developmental 
      transition, whereas in plants, they forestall developmental transitions by 
      repressing mRNAs that act later. We propose that, by preventing precocious 
      expression of differentiation-promoting transcription factors, miRNAs enable 
      proper embryonic patterning.
FAU - Nodine, Michael D
AU  - Nodine MD
AD  - Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA.
FAU - Bartel, David P
AU  - Bartel DP
LA  - eng
GR  - F32 GM084656/GM/NIGMS NIH HHS/United States
GR  - R01 GM067031/GM/NIGMS NIH HHS/United States
GR  - GM067031/GM/NIGMS NIH HHS/United States
GR  - F32GM084656/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (MicroRNAs)
RN  - 0 (SPL10 protein, Arabidopsis)
RN  - 0 (Transcription Factors)
RN  - EC 3.1.26.3 (DCL1 protein, Arabidopsis)
RN  - EC 3.1.26.3 (Ribonuclease III)
SB  - IM
CIN - Dev Cell. 2011 Jan 18;20(1):3-4. PMID: 21238918
MH  - Arabidopsis/genetics/*growth & development/*metabolism
MH  - Arabidopsis Proteins/*genetics/*metabolism
MH  - Cell Cycle Proteins/*genetics/*metabolism
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation, Plant
MH  - MicroRNAs/genetics/*metabolism
MH  - Phenotype
MH  - Ribonuclease III/*genetics/*metabolism
MH  - Seeds/growth & development/metabolism
MH  - Transcription Factors/metabolism
PMC - PMC2994041
EDAT- 2010/12/03 06:00
MHDA- 2010/12/22 06:00
PMCR- 2011/06/01
CRDT- 2010/12/03 06:00
PHST- 2010/12/03 06:00 [entrez]
PHST- 2010/12/03 06:00 [pubmed]
PHST- 2010/12/22 06:00 [medline]
PHST- 2011/06/01 00:00 [pmc-release]
AID - 24/23/2678 [pii]
AID - 10.1101/gad.1986710 [doi]
PST - ppublish
SO  - Genes Dev. 2010 Dec 1;24(23):2678-92. doi: 10.1101/gad.1986710.