PMID- 2112397
OWN - NLM
STAT- MEDLINE
DCOM- 19900713
LR  - 20141120
IS  - 0269-9370 (Print)
IS  - 0269-9370 (Linking)
VI  - 4
IP  - 3
DP  - 1990 Mar
TI  - Macrophage-HIV interaction: viral isolation and target cell tropism.
PG  - 221-8
AB  - Viral isolates were recovered by cocultivation on macrophage 
      colony-stimulatingfactor (MCSF)-treated monocyte target cells from peripheral 
      blood mononuclear cells (PBMCs) in 25 out of 27 patients seropositive or at risk 
      for HIV infection. Frequency of virus recovery was independent of the patient's 
      age, sex, numbers of CD4+ T cells, clinical stage or zidovudine (azidothymidine) 
      therapy. Sixteen out of 19 HIV isolates were serially passaged in MCSF- treated 
      monocytes. Five out of five virus isolates were also passaged in 
      phytohemagglutinin/interleukin-2 (PHA/IL-2)-treated lymphoblasts. In 
      lymphoblasts, no qualitative or quantitative differences were observed between 
      these isolates and human T-cell leukemia virus IIIB (HTLV-IIIB) for (1) release 
      of p24 antigen reverse transcriptase, and infectious virus, (2) induction of 
      typical cytopathic effects (cell syncytia in 3-10% of cells) and cell lysis, (3) 
      frequency of infected cells (5-20% of PBMC) as detected by in situ hybridization 
      for HIV RNA, (4) down-modulation of T cell plasma membrane CD4, and (5) site of 
      progeny virion assembly and budding (plasma membrane only with no 
      intracytoplasmic accumulation of virus). Progeny virus recovered from infected 
      lymphoblasts was fully infectious for other lymphoblasts, but failed to infect 
      MCSF-treated monocytes. Detailed analysis of target cell tropism among HIV 
      isolates showed that HIV isolated in monocytes infected both monocytes and 
      lymphoblasts; progeny virus isolated in lymphoblasts infected only T cells. HIV 
      interacts differently with monocytes and T cells. Understanding this interaction 
      may more clearly define both the pathogenesis of HIV disease and strategies for 
      therapeutic intervention.
FAU - Gendelman, H E
AU  - Gendelman HE
AD  - Henry M. Jackson Foundation for the Advancement of Military Medicine, Rockville, 
      Maryland.
FAU - Baca, L M
AU  - Baca LM
FAU - Husayni, H
AU  - Husayni H
FAU - Turpin, J A
AU  - Turpin JA
FAU - Skillman, D
AU  - Skillman D
FAU - Kalter, D C
AU  - Kalter DC
FAU - Orenstein, J M
AU  - Orenstein JM
FAU - Hoover, D L
AU  - Hoover DL
FAU - Meltzer, M S
AU  - Meltzer MS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - AIDS
JT  - AIDS (London, England)
JID - 8710219
RN  - 0 (Colony-Stimulating Factors)
RN  - 0 (Gene Products, gag)
RN  - 0 (HIV Core Protein p24)
RN  - 0 (Interleukin-2)
RN  - 0 (Phytohemagglutinins)
RN  - 0 (Viral Core Proteins)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Adult
MH  - Colony-Stimulating Factors/pharmacology
MH  - Female
MH  - Gene Products, gag/isolation & purification
MH  - HIV/*isolation & purification
MH  - HIV Core Protein p24
MH  - HIV Seropositivity/*microbiology
MH  - Humans
MH  - In Vitro Techniques
MH  - Interleukin-2/pharmacology
MH  - Lymphocytes/microbiology
MH  - Macrophage Colony-Stimulating Factor
MH  - Macrophages/*microbiology
MH  - Male
MH  - Middle Aged
MH  - Phytohemagglutinins/pharmacology
MH  - Viral Core Proteins/isolation & purification
EDAT- 1990/03/01 00:00
MHDA- 1990/03/01 00:01
CRDT- 1990/03/01 00:00
PHST- 1990/03/01 00:00 [pubmed]
PHST- 1990/03/01 00:01 [medline]
PHST- 1990/03/01 00:00 [entrez]
PST - ppublish
SO  - AIDS. 1990 Mar;4(3):221-8.