PMID- 21124018
OWN - NLM
STAT- MEDLINE
DCOM- 20110406
LR  - 20110210
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 132
IP  - 4
DP  - 2011
TI  - Cytotypes of Kirk's dik-dik (Madoqua kirkii,Bovidae) show multiple tandem 
      fusions.
PG  - 255-63
LID - 10.1159/000322483 [doi]
AB  - Madoqua kirkii, a miniature African antelope, is noted for extensive chromosomal 
      variation that has been categorized in four distinct cytotypes (A-D). In this 
      investigation, we analyzed the A cytotype (2n = 46, FN = 48) using a suite of 
      molecular cytogenetic approaches that entailed (i) whole chromosome and 
      subchromosomal painting by fluorescence in situ hybridization (FISH), (ii) the 
      study of Madoqua centromeric-specific DNA derived from pooled DNA obtained from 
      the centromeric regions of the acrocentric chromosomes, and (iii) DNA from the 
      telomere:centromere junctions of tandemly fused chromosomes. DNA from these 
      sources was used to probe for the persistence of interstitial satellite DNA and 
      residual centromeric sequences in the tandem and centric fusion junctions by PCR 
      and FISH. The analyses show centromeric sequences at two of the six tandem fusion 
      junctions. These data, and those of hybrid specimens (A x B cytotypes) in 
      conjunction with published information permitted an interpretation of the 
      probable sequence of chromosomal rearrangements among the M. kirkii cytotypes. We 
      discuss the findings in the context of chromosomal evolution in these antelopes, 
      and the implications that these hold for ex-situ breeding programs of the 
      species.
CI  - Copyright (c) 2010 S. Karger AG, Basel.
FAU - Cernohorska, H
AU  - Cernohorska H
AD  - Veterinary Research Institute, Brno, Czech Republic.
FAU - Kubickova, S
AU  - Kubickova S
FAU - Vahala, J
AU  - Vahala J
FAU - Robinson, T J
AU  - Robinson TJ
FAU - Rubes, J
AU  - Rubes J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101203
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA, Satellite)
SB  - IM
MH  - Animals
MH  - Antelopes/*genetics
MH  - Base Sequence
MH  - Centromere/*genetics
MH  - Chromosome Banding/methods
MH  - Chromosome Painting/methods
MH  - Chromosomes, Mammalian/genetics
MH  - DNA, Satellite/genetics
MH  - *Evolution, Molecular
MH  - Female
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Karyotyping/methods
MH  - Male
MH  - Telomere/genetics
EDAT- 2010/12/03 06:00
MHDA- 2011/04/07 06:00
CRDT- 2010/12/03 06:00
PHST- 2010/09/23 00:00 [accepted]
PHST- 2010/12/03 06:00 [entrez]
PHST- 2010/12/03 06:00 [pubmed]
PHST- 2011/04/07 06:00 [medline]
AID - 000322483 [pii]
AID - 10.1159/000322483 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2011;132(4):255-63. doi: 10.1159/000322483. Epub 2010 Dec 
      3.