PMID- 21125813
OWN - NLM
STAT- MEDLINE
DCOM- 20101230
LR  - 20131121
IS  - 0004-4172 (Print)
IS  - 0004-4172 (Linking)
VI  - 60
IP  - 10
DP  - 2010
TI  - Inhibition by folic acid of tumor necrosis factor alpha and apoptosis following 
      normothermic ischemia-reperfusion.
PG  - 621-6
LID - 10.1055/s-0031-1296336 [doi]
AB  - Folate is necessary for the production and maintenance of new cells and important 
      during periods of rapid cell division and growth. Tumor necrosis factor-alpha 
      (TNF-alpha) is known as a stimulant of apoptotic cell death. The present study 
      was aimed to evaluate the efficacy of folic acid (CAS 59-30-3) in prevention of 
      apoptosis by inhibiting TNF-alpha action in ischemia-reperfusion (I/R) induced 
      liver injury. Eighteen Wistar rats were subjected to 1 h of hepatic ischemia 
      followed by 3 h reperfusion and were divided into sham-operated control Group (I) 
      (n = 6), ischemia and reperfusion group administered 0.9% saline (5 ml/kg, p.o.) 
      for 7 days (II) (n = 6), folic acid treated group (1 mg/kg body weight/treated 
      daily by oral route for 7 days before induced ischemia-reperfusion maneuver) 
      (III) (n = 6). Hepatic damage in rats was assessed in terms of serum alanine 
      transaminases and aspartate transaminases. TNF-alpha concentration was measured 
      in serum by enzyme linked immuno assay. Necrosis and apoptosis were measured by 
      flow cytometry and fluorescence microscopy. Apoptotic marker Bcl-2 gene 
      expression was measured by reverse transcriptase polymerase chain reaction 
      (RTPCR) and Western Blot Analysis. Pathological changes were measured by 
      transmission electron microscopy (TEM). Serum alanine transaminase (ALT), 
      aspartate transaminase (AST) and TNF-alpha concentration increased significantly 
      (p < 0.05) in rats with I/R induced injury as compared to sham operated control 
      rats. Pretreatment with folic acid effectively counteracted the alternation in 
      hepatic enzymes. TEM, expression of Bcl-2 protein and flow cytometry studies 
      confirmed the restoration of cellular normalcy and accredits the cytoprotective 
      role of folic acid against I/R induced hepatic injury. The present study 
      demonstrated that elevated TNF-alpha activity directly related to apoptosis and 
      folic acid inhibits apoptosis by inhibiting the action of TNF-alpha.
FAU - Chattopadhyay, Pronobesh
AU  - Chattopadhyay P
AD  - Cellular and Microbiology Laboratory, College of Pharmacy, Institute of Foreign 
      Trade and Management, Moradabad, Uttar Pradesh, India. 
      chatto_pronobesh@rediffmail.com
FAU - Wahi, Arun Kumar
AU  - Wahi AK
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Arzneimittelforschung
JT  - Arzneimittel-Forschung
JID - 0372660
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 935E97BOY8 (Folic Acid)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 2.6.1.1 (Aspartate Aminotransferases)
RN  - EC 2.6.1.2 (Alanine Transaminase)
SB  - IM
MH  - Alanine Transaminase/blood
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Aspartate Aminotransferases/blood
MH  - Blotting, Western
MH  - Flow Cytometry
MH  - Folic Acid/*pharmacology
MH  - Gene Expression/drug effects
MH  - Genes, bcl-2/drug effects
MH  - Glucosephosphate Dehydrogenase/metabolism
MH  - Hepatocytes/drug effects
MH  - Male
MH  - Microscopy, Electron, Transmission
MH  - Microscopy, Fluorescence
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion Injury/*pathology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/*antagonists & inhibitors/blood/*pharmacology
EDAT- 2010/12/04 06:00
MHDA- 2010/12/31 06:00
CRDT- 2010/12/04 06:00
PHST- 2010/12/04 06:00 [entrez]
PHST- 2010/12/04 06:00 [pubmed]
PHST- 2010/12/31 06:00 [medline]
AID - 10.1055/s-0031-1296336 [doi]
PST - ppublish
SO  - Arzneimittelforschung. 2010;60(10):621-6. doi: 10.1055/s-0031-1296336.