PMID- 2112599
OWN - NLM
STAT- MEDLINE
DCOM- 19900716
LR  - 20091119
IS  - 0022-3395 (Print)
IS  - 0022-3395 (Linking)
VI  - 76
IP  - 3
DP  - 1990 Jun
TI  - Infections in mice with tachyzoites and bradyzoites of Neospora caninum 
      (Protozoa: Apicomplexa).
PG  - 410-3
AB  - Tachyzoites of 2 isolates of Neospora caninum (NC-1 and NC-2) were inoculated 
      subcutaneously (s.c.), intraperitoneally (i.p.), or orally into mice to compare 
      the effects of route of inoculation on pathogenicity. Mice developed more severe 
      disease, and disease occurred sooner when inoculated with the NC-1 isolate 
      compared to the NC-2 isolate. Deaths occurred earlier in mice inoculated i.p. 
      with either isolate. Mice inoculated orally or s.c. with tachyzoites responded 
      similarly to infection. Tissue cysts of the NC-2 isolate produced infections in 
      mice following oral or s.c. inoculation. Lesions seen in mice inoculated with 
      tachyzoites or bradyzoites were primarily acute pneumonia, myositis, 
      encephalitis, ganglioradiculoneuritis, and pancreatitis. In vitro studies 
      demonstrated that tachyzoites of both isolates were killed by incubation in 
      pepsin-HCl solution but not 1% trypsin solution. Bradyzoites of the NC-2 isolate 
      were able to withstand treatment with pepsin-HCl solution.
FAU - Lindsay, D S
AU  - Lindsay DS
AD  - U. S. Department of Agriculture, Livestock and Poultry Sciences Institute, 
      BARC-East, Beltsville, Maryland 20705.
FAU - Dubey, J P
AU  - Dubey JP
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Parasitol
JT  - The Journal of parasitology
JID - 7803124
RN  - EC 3.4.21.4 (Trypsin)
RN  - EC 3.4.23.1 (Pepsin A)
SB  - IM
MH  - Animals
MH  - Brain/parasitology
MH  - Disease Models, Animal
MH  - Eukaryota/drug effects
MH  - Female
MH  - Mice
MH  - Pepsin A/pharmacology
MH  - Protozoan Infections/parasitology/*pathology
MH  - Trypsin/pharmacology
EDAT- 1990/06/01 00:00
MHDA- 1990/06/01 00:01
CRDT- 1990/06/01 00:00
PHST- 1990/06/01 00:00 [pubmed]
PHST- 1990/06/01 00:01 [medline]
PHST- 1990/06/01 00:00 [entrez]
PST - ppublish
SO  - J Parasitol. 1990 Jun;76(3):410-3.