PMID- 21126330
OWN - NLM
STAT- MEDLINE
DCOM- 20110318
LR  - 20211020
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 7
DP  - 2010 Dec 2
TI  - Detection of porcine parvovirus using a taqman-based real-time pcr with primers 
      and probe designed for the NS1 gene.
PG  - 353
LID - 10.1186/1743-422X-7-353 [doi]
AB  - A TaqMan-based real-time polymerase chain reaction (PCR) assay was devised for 
      the detection of porcine parvovirus (PPV). Two primers and a TaqMan probe for the 
      non-structural protein NS1 gene were designed. The detection limit was 1 x 10(2) 
      DNA copies/muL, and the assay was linear in the range of 1 x 10(2) to 1 x 10(9) 
      copies/muL. There was no cross-reaction with porcine circovirus 2 (PCV2), porcine 
      reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), 
      classical swine fever virus (CSFV), or Japanese encephalitis virus (JEV). The 
      assay was specific and reproducible. In 41 clinical samples, PPV was detected in 
      32 samples with the real-time PCR assay and in only 11 samples with a 
      conventional PCR assay. The real-time assay using the TaqMan-system can therefore 
      be practically used for studying the epidemiology and management of PPV.
FAU - Song, Cuiping
AU  - Song C
AD  - Division of Swine Infectious Disease, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute of CAAS, Harbin, China 
      150001.
FAU - Zhu, Chao
AU  - Zhu C
FAU - Zhang, Chaofan
AU  - Zhang C
FAU - Cui, Shangjin
AU  - Cui S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101202
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 0 (DNA Primers)
RN  - 0 (DNA Probes)
RN  - 0 (NS1 protein, parvovirus)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Animals
MH  - DNA Primers/chemistry
MH  - DNA Probes/chemistry
MH  - Humans
MH  - Parvovirus, Porcine/*genetics
MH  - Polymerase Chain Reaction
MH  - Reproducibility of Results
MH  - *Reverse Transcriptase Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Swine Diseases/diagnosis/virology
MH  - Viral Nonstructural Proteins/*genetics
PMC - PMC3014914
EDAT- 2010/12/04 06:00
MHDA- 2011/03/19 06:00
PMCR- 2010/12/02
CRDT- 2010/12/04 06:00
PHST- 2010/06/02 00:00 [received]
PHST- 2010/12/02 00:00 [accepted]
PHST- 2010/12/04 06:00 [entrez]
PHST- 2010/12/04 06:00 [pubmed]
PHST- 2011/03/19 06:00 [medline]
PHST- 2010/12/02 00:00 [pmc-release]
AID - 1743-422X-7-353 [pii]
AID - 10.1186/1743-422X-7-353 [doi]
PST - epublish
SO  - Virol J. 2010 Dec 2;7:353. doi: 10.1186/1743-422X-7-353.