PMID- 21127704
OWN - NLM
STAT- MEDLINE
DCOM- 20110329
LR  - 20211020
IS  - 1110-7251 (Electronic)
IS  - 1110-7243 (Print)
IS  - 1110-7243 (Linking)
VI  - 2011
DP  - 2011
TI  - Isolation of BAC clones containing conserved genes from libraries of three 
      distantly related moths: a useful resource for comparative genomics of 
      Lepidoptera.
PG  - 165894
LID - 10.1155/2011/165894 [doi]
LID - 165894
AB  - Lepidoptera, butterflies and moths, is the second largest animal order and 
      includes numerous agricultural pests. To facilitate comparative genomics in 
      Lepidoptera, we isolated BAC clones containing conserved and putative single-copy 
      genes from libraries of three pests, Heliothis virescens, Ostrinia nubilalis, and 
      Plutella xylostella, harboring the haploid chromosome number, n = 31, which are 
      not closely related with each other or with the silkworm, Bombyx mori, (n = 28), 
      the sequenced model lepidopteran. A total of 108-184 clones representing 101-182 
      conserved genes were isolated for each species. For 79 genes, clones were 
      isolated from more than two species, which will be useful as common markers for 
      analysis using fluorescence in situ hybridization (FISH), as well as for 
      comparison of genome sequence among multiple species. The PCR-based clone 
      isolation method presented here is applicable to species which lack a sequenced 
      genome but have a significant collection of cDNA or EST sequences.
FAU - Yasukochi, Yuji
AU  - Yasukochi Y
AD  - Insect Genome Research Unit, National Institute of Agrobiological Sciences, 
      Tsukuba, Ibaraki, Japan. yaskoch@affrc.go.jp
FAU - Tanaka-Okuyama, Makiko
AU  - Tanaka-Okuyama M
FAU - Kamimura, Manabu
AU  - Kamimura M
FAU - Nakano, Ryo
AU  - Nakano R
FAU - Naito, Yota
AU  - Naito Y
FAU - Ishikawa, Yukio
AU  - Ishikawa Y
FAU - Sahara, Ken
AU  - Sahara K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101125
PL  - United States
TA  - J Biomed Biotechnol
JT  - Journal of biomedicine & biotechnology
JID - 101135740
SB  - IM
MH  - Animals
MH  - Chromosomes, Artificial, Bacterial/*genetics
MH  - Cloning, Molecular
MH  - Expressed Sequence Tags
MH  - *Gene Library
MH  - Genes, Insect
MH  - Genome, Insect
MH  - Genomics/*methods
MH  - Moths/*genetics
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Species Specificity
PMC - PMC2992816
EDAT- 2010/12/04 06:00
MHDA- 2011/03/30 06:00
PMCR- 2010/11/25
CRDT- 2010/12/04 06:00
PHST- 2010/06/30 00:00 [received]
PHST- 2010/10/25 00:00 [accepted]
PHST- 2010/12/04 06:00 [entrez]
PHST- 2010/12/04 06:00 [pubmed]
PHST- 2011/03/30 06:00 [medline]
PHST- 2010/11/25 00:00 [pmc-release]
AID - 10.1155/2011/165894 [doi]
PST - ppublish
SO  - J Biomed Biotechnol. 2011;2011:165894. doi: 10.1155/2011/165894. Epub 2010 Nov 
      25.