PMID- 21128635
OWN - NLM
STAT- MEDLINE
DCOM- 20110329
LR  - 20220321
IS  - 1520-5851 (Electronic)
IS  - 0013-936X (Linking)
VI  - 45
IP  - 2
DP  - 2011 Jan 15
TI  - A new process for efficiently producing methane from waste activated sludge: 
      alkaline pretreatment of sludge followed by treatment of fermentation liquid in 
      an EGSB reactor.
PG  - 803-8
LID - 10.1021/es102696d [doi]
AB  - In the literature the production of methane from waste activated sludge (WAS) was 
      usually conducted in a continuous stirred tank reactor (CSTR) after sludge was 
      pretreated. It was reported in our previous publication that compared with other 
      pretreatment methods the methane production in CSTR could be significantly 
      enhanced when sludge was pretreated by NaOH at pH 10 for 8 days. In order to 
      further improve methane production, this study reported a new process for 
      efficiently producing methane from sludge, that is, sludge was fermented at pH 10 
      for 8 days, which was adjusted by Ca(OH)(2), and then the fermentation liquid was 
      treated in an expanded granular sludge bed (EGSB) for methane generation. First, 
      for comparing the methane production observed in this study with that reported in 
      the literature, the conventional operational model was applied to produce methane 
      from the pH 10 pretreated sludge, that is, directly using the pH 10 pretreated 
      sludge to produce methane in a CSTR. It was observed that the maximal methane 
      production was only 0.61 m(3)CH(4)/m(3)-reactor/day. Then, the use of 
      fermentation liquid of pH 10 pretreated sludge to produce methane in the reactors 
      of up-flow anaerobic sludge bed (UASB), anaerobic sequencing batch reactor (ASBR) 
      and EGSB was compared. The maximal methane production in UASB, ASBR, and EGSB 
      reached 1.41, 3.01, and 12.43 m(3)CH(4)/m(3)-reactor/day, respectively. Finally, 
      the mechanisms for EGSB exhibiting remarkably higher methane production were 
      investigated by enzyme, adenosine-triphosphate (ATP), scanning electron 
      microscope (SEM) and fluorescence in situ hybridization (FISH) analyses. It was 
      found that the granular sludge in EGSB had the highest conversion efficiency of 
      acetic acid to methane, and the greatest activity of hydrolysis and acidification 
      enzymes and general physiology with much more Methanosarcinaceae.
FAU - Zhang, Dong
AU  - Zhang D
AD  - State Key Laboratory of Pollution Control and Resources Reuse, School of 
      Environmental Science and Engineering, Tongji University, Shanghai, China.
FAU - Chen, Yinguang
AU  - Chen Y
FAU - Zhao, Yuxiao
AU  - Zhao Y
FAU - Ye, Zhengxiang
AU  - Ye Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101203
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (Sewage)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - OP0UW79H66 (Methane)
RN  - Q40Q9N063P (Acetic Acid)
SB  - IM
MH  - Acetic Acid/metabolism
MH  - Adenosine Triphosphate/metabolism
MH  - Anaerobiosis
MH  - Biodegradation, Environmental
MH  - Bioreactors/*microbiology
MH  - Fermentation
MH  - Hydrogen-Ion Concentration
MH  - Methane/analysis/*metabolism
MH  - Methanosarcinaceae/classification/metabolism
MH  - Microscopy, Electron, Scanning
MH  - Sewage/*microbiology
MH  - Waste Disposal, Fluid/*methods
EDAT- 2010/12/07 06:00
MHDA- 2011/03/30 06:00
CRDT- 2010/12/07 06:00
PHST- 2010/12/07 06:00 [entrez]
PHST- 2010/12/07 06:00 [pubmed]
PHST- 2011/03/30 06:00 [medline]
AID - 10.1021/es102696d [doi]
PST - ppublish
SO  - Environ Sci Technol. 2011 Jan 15;45(2):803-8. doi: 10.1021/es102696d. Epub 2010 
      Dec 3.