PMID- 21128778
OWN - NLM
STAT- MEDLINE
DCOM- 20110106
LR  - 20210527
IS  - 1543-2165 (Electronic)
IS  - 0003-9985 (Linking)
VI  - 134
IP  - 12
DP  - 2010 Dec
TI  - Utilization of fluorescence in situ hybridization in the diagnosis of 230 
      mesenchymal neoplasms: an institutional experience.
PG  - 1797-803
AB  - CONTEXT: Mesenchymal neoplasms harbor characteristic translocations and 
      amplification of gene regions amenable to evaluation by fluorescence in situ 
      hybridization (FISH). OBJECTIVE: To determine the utility of FISH in the 
      diagnosis of mesenchymal neoplasms. DESIGN: Two hundred thirty soft tissue cases 
      analyzed by FISH were reviewed retrospectively. RESULTS: Morphologic patterns 
      where FISH was used included high-grade round cell sarcomas (n  =  67), 
      nonmyogenic spindle cell sarcomas (n  =  40), low-grade myxoid neoplasms (n  =  
      34), adipocytic neoplasms (n  =  20), and melanocytic neoplasms (n  =  19). Fifty 
      cases did not fit into the previously mentioned categories. SYT FISH (96% of 
      monophasic synovial sarcomas were positive; 0% of malignant peripheral nerve 
      sheath tumor were positive) and DDIT3 FISH (100% of myxoid/round cell 
      liposarcomas; no other neoplasm positive) were very sensitive and specific. EWSR1 
      FISH was very sensitive and specific in the differential diagnosis of melanocytic 
      neoplasms (88% of clear cell sarcomas were positive; all melanomas were 
      negative). EWSR1 FISH was sensitive among high-grade round cell sarcomas 
      (positive in 100% of desmoplastic small round cell tumors and 96% of Ewing 
      sarcoma/primitive neuroectodermal tumors) but not specific because clear cell 
      sarcoma, extraskeletal myxoid chondrosarcoma, and a subset of round cell 
      liposarcomas also harbor rearrangements of EWSR1. FUS FISH was very sensitive in 
      detecting low-grade fibromyxoid sarcomas (91% positive) but not specific because 
      most myxoid/round cell liposarcomas also contain rearrangements of FUS. All 
      atypical lipomatous tumors were positive for amplification of MDM2, whereas all 
      lipomas were negative. FOXO1A FISH was positive in  approximately 70% of cases of alveolar 
      rhabdomyosarcoma. CONCLUSION: FISH is a useful adjunct in the diagnosis of 
      mesenchymal neoplasms.
FAU - Tanas, Munir R
AU  - Tanas MR
AD  - Department of Pathology, Cleveland Clinic, Ohio, USA.
FAU - Rubin, Brian P
AU  - Rubin BP
FAU - Tubbs, Raymond R
AU  - Tubbs RR
FAU - Billings, Steven D
AU  - Billings SD
FAU - Downs-Kelly, Erinn
AU  - Downs-Kelly E
FAU - Goldblum, John R
AU  - Goldblum JR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Arch Pathol Lab Med
JT  - Archives of pathology & laboratory medicine
JID - 7607091
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Biomarkers, Tumor/metabolism
MH  - Chromosome Aberrations
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Melanoma/*diagnosis/genetics/metabolism
MH  - Molecular Diagnostic Techniques
MH  - Neoplasms, Adipose Tissue/*diagnosis/genetics/metabolism
MH  - Sarcoma/*diagnosis/genetics/metabolism
MH  - Soft Tissue Neoplasms/*diagnosis/genetics/metabolism
EDAT- 2010/12/07 06:00
MHDA- 2011/01/07 06:00
CRDT- 2010/12/07 06:00
PHST- 2010/12/07 06:00 [entrez]
PHST- 2010/12/07 06:00 [pubmed]
PHST- 2011/01/07 06:00 [medline]
AID - 10.1043/2009-0571-OAR.1 [pii]
AID - 10.5858/2009-0571-OAR.1 [doi]
PST - ppublish
SO  - Arch Pathol Lab Med. 2010 Dec;134(12):1797-803. doi: 10.5858/2009-0571-OAR.1.