PMID- 21129263
OWN - NLM
STAT- MEDLINE
DCOM- 20111013
LR  - 20161018
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 18
IP  - 5
DP  - 2010 Oct
TI  - [Impact of cyclosporine A on the expression of T-bet, GATA-3, relevant signal 
      transduction molecules, cytokine and Th1/Th2 balance in patients with chronic 
      aplastic anemia].
PG  - 1211-9
AB  - The study was aimed to explore the effects of T-bet (T-box expressed in T cell), 
      GATA-3(GATA binding protein 3) and relevant signal transduction pathways on the 
      immune-related pathogenesis of chronic aplastic anemia (CAA), and to investigate 
      the immunological regulation mechanism in the treatment of CAA by using 
      cyclosporine A (CsA) at the level of Th cell imbalance, transcriptional factors, 
      and relevant signal pathways. The real-time fluorescent quantitative polymerase 
      chain reaction (real-time FQ-PCR) was used to determine the mRNA expression of 
      T-bet, GATA-3, signal transducers and activators of transcription 4 (STAT4) and 
      signal transducers and activators of transcription 6 (STAT6) in peripheral blood 
      mononuclear cell (PBMNC) of CAA patients before and after treatment with CsA; the 
      flow cytometry (FCM) and enzyme linked immunosorbent assay (ELISA) were used to 
      determine the Th1/Th2 proportion in peripheral blood, and levels of IFN-gamma, IL-12 
      and IL-4 in PBMNC-cultured supernatant. Healthy people were included to test the 
      above indexes. The results showed that the mRNA expression of PBMNC T-bet, STAT4, 
      T-bet/GATA-3 ratio, Th1 proportion, Th1/Th2 ratio and levels of IFN-gamma and IL-12 
      in PBMNC-cultured supernatant of CAA patients were significantly higher than 
      those of healthy people (p < 0.01). After treating with CsA for 6 months of CsA 
      treatment, expression of T-bet, STAT4, T-bet/GATA-3 ratio, Th1 proportion, IFN-gamma 
      and IL-12 levels were lower than before, however, the expression of T-bet, STAT4, 
      T-bet/GATA-3 ratio, Th1 proportion and IFN-gamma had not been reduced to normal 
      state. Compared to healthy people, no significant difference existed in the mRNA 
      expression of GATA-3, STAT6, Th2 proportion, as well as level of IL-4 before and 
      after treatment (p>0.05). It is concluded that the abnormal activation of 
      IFN-gamma/T-bet and IL-12/STAT4 pathways, as well as Th balance deviating to Th1 
      excursion play vital roles in the immunological pathogenesis of AA. CsA lowers 
      the abnormal activation of IFN-gamma/T-bet and IL-12/STAT4 pathways to correct Th1 
      hyperpolarization, which may reduce the abnormally activated cell-mediated 
      immunity and relax hematopoietic depression of AA patients.
FAU - Li, Jun
AU  - Li J
AD  - Department of Hematology, Jiangsu Provincial Hospital of Chinese Traditional 
      Medicine, Nanjing University of Chinese Traditional Medicine, Nanjing 210029, 
      Jiangsu Province, China.
FAU - Zhou, Yong-Ming
AU  - Zhou YM
FAU - Hu, Ming-Hui
AU  - Hu MH
FAU - Sun, Wei-Ling
AU  - Sun WL
FAU - Xue, Zhi-Zhong
AU  - Xue ZZ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 0 (GATA3 Transcription Factor)
RN  - 0 (GATA3 protein, human)
RN  - 0 (IL4 protein, human)
RN  - 0 (STAT4 Transcription Factor)
RN  - 0 (STAT4 protein, human)
RN  - 0 (STAT6 Transcription Factor)
RN  - 0 (STAT6 protein, human)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (T-box transcription factor TBX21)
RN  - 187348-17-0 (Interleukin-12)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 83HN0GTJ6D (Cyclosporine)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Anemia, Aplastic/*drug therapy/immunology/metabolism
MH  - Case-Control Studies
MH  - Cyclosporine/*pharmacology
MH  - Female
MH  - GATA3 Transcription Factor/*metabolism
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Interleukin-12/metabolism
MH  - Interleukin-4/metabolism
MH  - Male
MH  - Middle Aged
MH  - STAT4 Transcription Factor/metabolism
MH  - STAT6 Transcription Factor/metabolism
MH  - Signal Transduction
MH  - T-Box Domain Proteins/*metabolism
MH  - Th1 Cells/*drug effects
MH  - Th2 Cells/*drug effects
MH  - Young Adult
EDAT- 2010/12/07 06:00
MHDA- 2011/10/14 06:00
CRDT- 2010/12/07 06:00
PHST- 2010/12/07 06:00 [entrez]
PHST- 2010/12/07 06:00 [pubmed]
PHST- 2011/10/14 06:00 [medline]
AID - 1009-2137(2010)05-1211-09 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2010 Oct;18(5):1211-9.