PMID- 21131977
OWN - NLM
STAT- MEDLINE
DCOM- 20110211
LR  - 20211020
IS  - 1545-9985 (Electronic)
IS  - 1545-9993 (Print)
IS  - 1545-9985 (Linking)
VI  - 18
IP  - 1
DP  - 2011 Jan
TI  - Transcription of functionally related constitutive genes is not coordinated.
PG  - 27-34
LID - 10.1038/nsmb.1934 [doi]
AB  - Expression of an individual gene can vary considerably among genetically 
      identical cells because of stochastic fluctuations in transcription. However, 
      proteins comprising essential complexes or pathways have similar abundances and 
      lower variability. It is not known whether coordination in the expression of 
      subunits of essential complexes occurs at the level of transcription, mRNA 
      abundance or protein expression. To directly measure the level of coordination in 
      the expression of genes, we used highly sensitive fluorescence in situ 
      hybridization (FISH) to count individual mRNAs of functionally related and 
      unrelated genes within single Saccharomyces cerevisiae cells. Our results 
      revealed that transcript levels of temporally induced genes are highly correlated 
      in individual cells. In contrast, transcription of constitutive genes encoding 
      essential subunits of complexes is not coordinated because of stochastic 
      fluctuations. The coordination of these functional complexes therefore must occur 
      post-transcriptionally, and likely post-translationally.
FAU - Gandhi, Saumil J
AU  - Gandhi SJ
AD  - Department of Anatomy and Structural Biology, Albert Einstein College of 
      Medicine, Bronx, New York, USA.
FAU - Zenklusen, Daniel
AU  - Zenklusen D
FAU - Lionnet, Timothee
AU  - Lionnet T
FAU - Singer, Robert H
AU  - Singer RH
LA  - eng
GR  - R01 GM057071/GM/NIGMS NIH HHS/United States
GR  - R01 GM057071-12/GM/NIGMS NIH HHS/United States
GR  - GM 57071/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20101205
PL  - United States
TA  - Nat Struct Mol Biol
JT  - Nature structural & molecular biology
JID - 101186374
RN  - 0 (RNA, Messenger)
RN  - 0 (Saccharomyces cerevisiae Proteins)
SB  - IM
MH  - *Gene Expression Regulation
MH  - In Situ Hybridization, Fluorescence
MH  - *Models, Genetic
MH  - RNA, Messenger/metabolism
MH  - Saccharomyces cerevisiae/*genetics
MH  - Saccharomyces cerevisiae Proteins/*genetics
MH  - *Transcription, Genetic
PMC - PMC3058351
MID - NIHMS237678
EDAT- 2010/12/07 06:00
MHDA- 2011/02/12 06:00
PMCR- 2011/07/01
CRDT- 2010/12/07 06:00
PHST- 2010/02/04 00:00 [received]
PHST- 2010/09/17 00:00 [accepted]
PHST- 2010/12/07 06:00 [entrez]
PHST- 2010/12/07 06:00 [pubmed]
PHST- 2011/02/12 06:00 [medline]
PHST- 2011/07/01 00:00 [pmc-release]
AID - nsmb.1934 [pii]
AID - 10.1038/nsmb.1934 [doi]
PST - ppublish
SO  - Nat Struct Mol Biol. 2011 Jan;18(1):27-34. doi: 10.1038/nsmb.1934. Epub 2010 Dec 
      5.