PMID- 21132001
OWN - NLM
STAT- MEDLINE
DCOM- 20110715
LR  - 20221207
IS  - 1745-7262 (Electronic)
IS  - 1008-682X (Print)
IS  - 1008-682X (Linking)
VI  - 13
IP  - 2
DP  - 2011 Mar
TI  - Alteration of ERbeta gene RsaI polymorphism may contribute to reduced fertilization 
      rate and embryonic developmental competence.
PG  - 317-21
LID - 10.1038/aja.2010.150 [doi]
AB  - This paper aims to determine the possible role of estrogen receptor-beta (ERbeta) gene 
      RsaI polymorphism on sperm fertility and early embryonic development in humans. 
      Three groups of Chinese men were recruited: in vitro fertilization (IVF) group, 
      including 374 couples who underwent conventional IVF; intracytoplasmic sperm 
      injection (ICSI) group, including 294 couples who underwent an ICSI procedure 
      using ejaculated sperm; and azoospermic group, consisting of 197 couples who 
      underwent ICSI using either testis or epididymis sperm. RsaI polymorphism in the 
      ERbeta gene was detected by polymerase chain reaction (PCR)-restriction fragment 
      length polymorphism technique; fertilization and high-quality embryo rates were 
      evaluated for each group. In each group, no significant differences were found in 
      the overall rates of fertilization and high-quality embryos among GG, AG and AA 
      genotypes. However, the proportion of cycles possessing a satisfactory 
      high-quality embryo rate with the AA genotype was significantly lower than that 
      in the wild-type GG genotype from each group. These results demonstrated that 
      sperm possessing the ERbeta RsaI A genotype may have reduced fertilization ability 
      and decreased early embryonic developmental potential, which could directly or 
      indirectly contribute to the low fertilization rate and early embryonic 
      developmental arrest in some cases.
FAU - Zhang, Qiu-Fang
AU  - Zhang QF
AD  - Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking 
      University Third Hospital, Beijing 1000191, China.
FAU - Feng, Huai-Liang
AU  - Feng HL
FAU - Zhao, Lan
AU  - Zhao L
FAU - Liu, Ping
AU  - Liu P
FAU - Li, Li
AU  - Li L
FAU - Yan, Jie
AU  - Yan J
FAU - Qiao, Jie
AU  - Qiao J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101206
PL  - China
TA  - Asian J Androl
JT  - Asian journal of andrology
JID - 100942132
RN  - 0 (DNA Primers)
RN  - 0 (Estrogen Receptor beta)
SB  - IM
MH  - Asian People/genetics
MH  - Azoospermia/genetics/physiopathology/therapy
MH  - Base Sequence
MH  - China
MH  - DNA Primers/genetics
MH  - Embryonic Development/*genetics/*physiology
MH  - Estrogen Receptor beta/*genetics/*physiology
MH  - Female
MH  - Fertility/*genetics/*physiology
MH  - Fertilization in Vitro
MH  - Gene Frequency
MH  - Genotype
MH  - Humans
MH  - Infertility, Male/*genetics/*physiopathology/therapy
MH  - Male
MH  - Polymorphism, Restriction Fragment Length
MH  - Pregnancy
MH  - Sperm Injections, Intracytoplasmic
PMC - PMC3739193
EDAT- 2010/12/07 06:00
MHDA- 2011/07/16 06:00
PMCR- 2011/03/01
CRDT- 2010/12/07 06:00
PHST- 2010/12/07 06:00 [entrez]
PHST- 2010/12/07 06:00 [pubmed]
PHST- 2011/07/16 06:00 [medline]
PHST- 2011/03/01 00:00 [pmc-release]
AID - aja2010150 [pii]
AID - 10.1038/aja.2010.150 [doi]
PST - ppublish
SO  - Asian J Androl. 2011 Mar;13(2):317-21. doi: 10.1038/aja.2010.150. Epub 2010 Dec 
      6.