PMID- 21134098
OWN - NLM
STAT- MEDLINE
DCOM- 20110801
LR  - 20181201
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Linking)
VI  - 13
IP  - 3
DP  - 2011 Mar
TI  - Novel groups of Gammaproteobacteria catalyse sulfur oxidation and carbon fixation 
      in a coastal, intertidal sediment.
PG  - 758-74
LID - 10.1111/j.1462-2920.2010.02380.x [doi]
AB  - The oxidation of hydrogen sulfide is essential to sulfur cycling in marine 
      habitats. However, the role of microbial sulfur oxidation in marine sediments and 
      the microorganisms involved are largely unknown, except for the filamentous, 
      mat-forming bacteria. In this study we explored the diversity, abundance and 
      activity of sulfur-oxidizing prokaryotes (SOP) in sulfidic intertidal sediments 
      using 16S rRNA and functional gene sequence analyses, fluorescence in situ 
      hybridization (FISH) and microautoradiography. The 16S rRNA gene analysis 
      revealed that distinct clades of uncultured Gammaproteobacteria are important SOP 
      in the tidal sediments. This was supported by the dominance of 
      gammaproteobacterial sequences in clone libraries of genes encoding the reverse 
      dissimilatory sulfite reductase (rDSR) and the adenosine phosphosulfate reductase 
      (APR). Numerous sequences of all three genes grouped with uncultured autotrophic 
      SOP. Accordingly, Gammaproteobacteria accounted for 40-70% of all (1)(4)CO(2) 
      -incorporating cells in surface sediments as shown by microautoradiography. 
      Furthermore, phylogenetic analysis of all three genes consistently suggested a 
      discrete population of SOP that was most closely related to the sulfur-oxidizing 
      endosymbionts of the tubeworm Oligobrachia spp. FISH showed that members of this 
      population (WS-Gam209 group) were abundant, reaching up to 1.3 x 10(8) cells ml(-)(1) 
      (4.6% of all cells). Approximately 25% of this population incorporated CO(2), 
      consistent with a chemolithoautotrophic metabolism most likely based on sulfur 
      oxidation. Thus, we hypothesize that novel, gammaproteobacterial SOP attached to 
      sediment particles may play a more important role for sulfide removal and primary 
      production in marine sediments than previously assumed.
CI  - (c) 2010 Society for Applied Microbiology and Blackwell Publishing Ltd.
FAU - Lenk, Sabine
AU  - Lenk S
AD  - Max Planck Institute for Marine Microbiology, Bremen, Germany.
FAU - Arnds, Julia
AU  - Arnds J
FAU - Zerjatke, Katrice
AU  - Zerjatke K
FAU - Musat, Niculina
AU  - Musat N
FAU - Amann, Rudolf
AU  - Amann R
FAU - Mussmann, Marc
AU  - Mussmann M
LA  - eng
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PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101206
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sulfides)
RN  - 70FD1KFU70 (Sulfur)
RN  - EC 1.8.99.3 (Hydrogensulfite Reductase)
SB  - IM
MH  - Biocatalysis
MH  - *Carbon Cycle
MH  - Gammaproteobacteria/classification/genetics/*metabolism
MH  - Genes, rRNA
MH  - Geologic Sediments/*microbiology
MH  - Hydrogensulfite Reductase/genetics
MH  - Molecular Sequence Data
MH  - Oxidation-Reduction
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sulfides/metabolism
MH  - Sulfur/*metabolism
EDAT- 2010/12/08 06:00
MHDA- 2011/08/02 06:00
CRDT- 2010/12/08 06:00
PHST- 2010/12/08 06:00 [entrez]
PHST- 2010/12/08 06:00 [pubmed]
PHST- 2011/08/02 06:00 [medline]
AID - 10.1111/j.1462-2920.2010.02380.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2011 Mar;13(3):758-74. doi: 10.1111/j.1462-2920.2010.02380.x. 
      Epub 2010 Dec 6.