PMID- 21136334
OWN - NLM
STAT- MEDLINE
DCOM- 20110201
LR  - 20180802
IS  - 1424-3997 (Electronic)
IS  - 0036-7672 (Linking)
VI  - 140
DP  - 2010
TI  - Suppressive effects of black seed oil on ovalbumin induced acute lung remodeling 
      in E3 rats.
PG  - w13128
LID - 10.4414/smw.2010.13128 [doi]
AB  - OBJECTIVE: Black seed oil (BSO) is widely used as a traditional medicine for 
      asthma and other inflammatory diseases. The aim of this study is to evaluate the 
      effects of BSO on ovalbumin (OVA) induced acute lung remodelling in E3 inbred 
      rats. METHOD: Rats were divided into three groups; Control, OVA and BSO. The rats 
      were intraperitoneally sensitised and challenged intranasally with OVA and 
      treated intraperitoneally with pure BSO for seven days. The collagen deposition 
      and other pathological alteration were determined by Masson's trichrome, PAS and 
      HE staining. Activity of arginase, ornithine decarboxylase (ODC) and proline 
      level was determined by spectrophotometry, and polyamine by HPLC. The mRNA 
      expression of arginase capital I, Ukrainian, endothelin1 (Edn1), matrix metallopeptidase 3 (MMP3) 
      and growth factors was determined by real time RT-PCR. RESULTS: Massive 
      inflammation and characteristics of lung remodelling including collagen 
      deposition, goblet cell hyperplasia and proline level were observed in the lungs 
      of OVA exposed rats. Administration of BSO in the OVA exposed rats suppressed the 
      inflammatory cells infiltration, goblet cell hyperplasia and collagen deposition. 
      The activity of total arginase and ODC; proline and polyamine level was decreased 
      in the lung homogenate of BSO treated rats. Furthermore, BSO abrogated the mRNA 
      expression of Edn1, MMP3, transforming growth factor beta (TGF-beta), fibroblast 
      growth factor 2 (FGF2) and vascular epidermal growth factor (VEGF) in the lungs 
      of OVA challenged rats. CONCLUSION: Administration of BSO significantly reduced 
      the level of allergen induced lung remodelling. The effect of BSO on lung 
      remodelling is probably mediated by the inhibition of arginase pathways and the 
      expression of Edn1, MMP3 and growth factors. Our findings suggest that BSO might 
      have useful implications in the treatment and future research into 
      allergen-induced lung remodelling.
FAU - Raza Asim, M B
AU  - Raza Asim MB
AD  - Department of Genetics and Molecular Biology, Xi'an Jiaotong University College 
      of Medicine, Xi'an, Shaanxi, PR China.
FAU - Shahzad, Muhammad
AU  - Shahzad M
FAU - Yang, Xudong
AU  - Yang X
FAU - Sun, Qingzhu
AU  - Sun Q
FAU - Zhang, Fujun
AU  - Zhang F
FAU - Han, Yan
AU  - Han Y
FAU - Lu, Shemin
AU  - Lu S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101207
PL  - Switzerland
TA  - Swiss Med Wkly
JT  - Swiss medical weekly
JID - 100970884
RN  - 0 (Plant Oils)
RN  - 9006-59-1 (Ovalbumin)
RN  - C2J9B08Q3I (caraway oil)
SB  - IM
MH  - Animals
MH  - Asthma/chemically induced/*complications
MH  - Female
MH  - Lung Diseases/*etiology/*prevention & control
MH  - Male
MH  - Ovalbumin/administration & dosage
MH  - Plant Oils/*therapeutic use
MH  - Rats
EDAT- 2010/12/08 06:00
MHDA- 2011/02/02 06:00
CRDT- 2010/12/08 06:00
PHST- 2010/12/08 06:00 [entrez]
PHST- 2010/12/08 06:00 [pubmed]
PHST- 2011/02/02 06:00 [medline]
AID - smw-13128 [pii]
AID - 10.4414/smw.2010.13128 [doi]
PST - epublish
SO  - Swiss Med Wkly. 2010 Dec 7;140:w13128. doi: 10.4414/smw.2010.13128. eCollection 
      2010.