PMID- 21141598
OWN - NLM
STAT- MEDLINE
DCOM- 20121001
LR  - 20101213
IS  - 1000-6834 (Print)
IS  - 1000-6834 (Linking)
VI  - 24
IP  - 3
DP  - 2008 Aug
TI  - [Roles of NRF2 regulating gamma-glutamylcysteine synthetase in lung of rats with 
      chronic obstructive pulmonary disease].
PG  - 339-42
AB  - AIM: To investigate the expression and relationship of gamma-glutamylcysteine 
      synthetase (gamma-GCS) and NF-E2-related factor2 (NRR2) in lung of rat with 
      chronic obstructive pulmonary disease (COPD)in order to elucidate the possible 
      important role of gamma-GCS and NRF2 in pathogenesis of COPD. METHODS: The rat 
      COPD model was established by intratracheal instillation of lipopolysaccharide 
      twice and exposed to cigarette smoke daily. The gamma-GCS activity was measured, 
      the expression of gamma-GCS mRNA in lung was examined by in situ hybridization 
      (ISH) and reverse transcription-polymerase chain reaction (RT-PCR), the protein 
      expressions of NRF2, gamma-GCS in lung were detected by immunohistochemical (IH) 
      and Western blot respectively. RESULTS: The gamma-GCS activity was higher in COPD 
      group than that in control group. The expressions of gamma-GCS mRNA in COPD group 
      was stronger than those in control group. ISH showed that the gamma-GCS mRNA was 
      expressed in alveolar epithelium and bronchial smooth muscle cell in COPD. The 
      protein expressions of NRF2, gamma-GCS were significantly higher than the control 
      group. IH showed that NRF2, gamma-GCS proteins were expressed in alveolar and 
      bronchial epithelium in the COPD group. There was a positive correlation between 
      NRF2 and gamma-GCS and gamma-GCS mRNA. CONCLUSION: NRF2 may play an important 
      role in the mechanism of COPD oxidative stress vis up-regulation of gamma-GCS.
FAU - Chen, Lin
AU  - Chen L
AD  - Department of Respiratory Medicine, Geriatric Hospital of Hunan Province, 
      Changsha 410001, China.
FAU - Dai, Ai-Guo
AU  - Dai AG
FAU - Hu, Rui-Cheng
AU  - Hu RC
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Ying Yong Sheng Li Xue Za Zhi
JT  - Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = 
      Chinese journal of applied physiology
JID - 9426407
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Nfe2l2 protein, rat)
RN  - EC 6.3.2.2 (Glutamate-Cysteine Ligase)
SB  - IM
MH  - Animals
MH  - Glutamate-Cysteine Ligase/*metabolism
MH  - Lung/*metabolism
MH  - Male
MH  - NF-E2-Related Factor 2/*metabolism
MH  - Oxidative Stress
MH  - Pulmonary Disease, Chronic Obstructive/*metabolism/*physiopathology
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
EDAT- 2008/08/01 00:00
MHDA- 2012/10/02 06:00
CRDT- 2010/12/15 06:00
PHST- 2010/12/15 06:00 [entrez]
PHST- 2008/08/01 00:00 [pubmed]
PHST- 2012/10/02 06:00 [medline]
PST - ppublish
SO  - Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2008 Aug;24(3):339-42.