PMID- 21142900
OWN - NLM
STAT- MEDLINE
DCOM- 20110316
LR  - 20181217
IS  - 1477-2566 (Electronic)
IS  - 1465-3249 (Linking)
VI  - 13
IP  - 1
DP  - 2011 Jan
TI  - Bone marrow-derived mesenchymal stromal cells support rat pancreatic islet 
      survival and insulin secretory function in vitro.
PG  - 19-29
LID - 10.3109/14653249.2010.518608 [doi]
AB  - BACKGROUND AIMS: Recent evidence has suggested that transplanted bone marrow 
      (BM)-derived mesenchymal stromal cells (MSC) are able to engraft and repair 
      non-hematopoietic tissues successfully, including central nervous system, renal, 
      pulmonary and skin tissue, and may possibly contribute to tissue regeneration. We 
      examined the cytoprotective effect of BM MSC on co-cultured, isolated pancreatic 
      islets. METHODS: Pancreatic islets and MSC isolated from Lewis rats were divided 
      into four experimental groups: (a) islets cultured alone (islet control); (b) 
      islets cultured in direct contact with MSC (IM-C); (c) islets co-cultured with 
      MSC in a Transwell system, which allows indirect cell contact through diffusible 
      media components (IM-I); and (d) MSC cultured alone (MSC control). The survival 
      and function of islets were measured morphologically and by analyzing insulin 
      secretion in response to glucose challenge. Cytokine profiles were determined 
      using a cytokine array and enzyme-linked immunosorbent assays. RESULTS: Islets 
      contact-cultured with MSC (IM-C) showed sustained survival and retention of 
      glucose-induced insulin secretory function. In addition, the levels of monocyte 
      chemoattractant protein-1 (MCP-1) and tumor necrosis factor-alpha (TNF-alpha) were 
      decreased, and tissue inhibitor of metalloproteinases-1 (TIMP-1) and vascular 
      endothelial growth factor (VEGF) levels were increased at 4 weeks in both the 
      IM-C and IM-I groups. CONCLUSIONS: These results indicate that contact co-culture 
      is a major factor that contributes to islet survival, maintenance of cell 
      morphology and insulin function. There might also be a synergic effect resulting 
      from the regulation of inflammatory cytokine production. We propose that BM MSC 
      are suitable for generating a microenvironment favorable for the repair and 
      longevity of pancreatic islets.
FAU - Jung, Eun-Jung
AU  - Jung EJ
AD  - Asan Institute for Life Science, Asan Medical Center, Seoul, Republic of Korea.
FAU - Kim, Song-Cheol
AU  - Kim SC
FAU - Wee, Yu-Mee
AU  - Wee YM
FAU - Kim, Yang-Hee
AU  - Kim YH
FAU - Choi, Monica Young
AU  - Choi MY
FAU - Jeong, Seong-Hee
AU  - Jeong SH
FAU - Lee, Jiyeon
AU  - Lee J
FAU - Lim, Dong-Gyun
AU  - Lim DG
FAU - Han, Duck-Jong
AU  - Han DJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cytotherapy
JT  - Cytotherapy
JID - 100895309
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Insulin)
RN  - 9007-92-5 (Glucagon)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology
MH  - Cell Adhesion
MH  - Cell Shape
MH  - Cell Survival
MH  - Coculture Techniques
MH  - Cytokines/metabolism
MH  - Glucagon/metabolism
MH  - Inflammation Mediators/metabolism
MH  - Insulin/*metabolism
MH  - Insulin Secretion
MH  - Islets of Langerhans/*cytology/*metabolism
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Phenotype
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Stromal Cells/cytology
EDAT- 2010/12/15 06:00
MHDA- 2011/03/17 06:00
CRDT- 2010/12/15 06:00
PHST- 2010/12/15 06:00 [entrez]
PHST- 2010/12/15 06:00 [pubmed]
PHST- 2011/03/17 06:00 [medline]
AID - S1465-3249(11)70477-7 [pii]
AID - 10.3109/14653249.2010.518608 [doi]
PST - ppublish
SO  - Cytotherapy. 2011 Jan;13(1):19-29. doi: 10.3109/14653249.2010.518608.