PMID- 21143018
OWN - NLM
STAT- MEDLINE
DCOM- 20110527
LR  - 20220321
IS  - 1437-4331 (Electronic)
IS  - 1434-6621 (Linking)
VI  - 49
IP  - 2
DP  - 2011 Feb
TI  - A new tool in the field of in-vitro diagnosis of allergy: preliminary results in 
      the comparison of ImmunoCAP(c) 250 with the ImmunoCAP(c) ISAC.
PG  - 277-80
LID - 10.1515/CCLM.2011.052 [doi]
AB  - BACKGROUND: The ImmunoCAP(c) ISAC allows for the determination of specific 
      immunoglobulin E (IgE) against 103 recombinant or purified allergen components in 
      a single analytical step. The aim of our study was to perform a comparison of the 
      specific IgE results measured with the microarray method to those obtained using 
      the traditional method of ImmunoCAP(c). METHODS: We selected 86 clinically relevant 
      patients on the basis of their specific IgE for recombinant allergens (ImmunoCAP(c) 
      250, Phadia). Also, we selected two patients with a high total IgE to evaluate 
      the non-specific binding of IgE. All samples were screened with the ImmunoCAP(c) 
      ISAC. Then, we compared the 555 specific IgE antibodies results provided by 
      ImmunoCAP(c) ISAC with the specific IgE levels obtained with ImmunoCAP(c) 250. 
      RESULTS: We observed that 82 of the 384 results found to be positive with 
      ImmunoCAP(c) were negative with ISAC(c) (concordance 78.65%). Of 171 negative results 
      obtained with ImmunoCAP(c), 11 were positive with ISAC(c) (concordance 93.57%). No 
      non-specific binding was observed. CONCLUSIONS: Our results show that the 
      ImmunoCAP(c) ISAC has good analytical performance when compared with the ImmunoCAP(c) 
      250 method. We did not observe any non-specific binding. However, better 
      sensitivity for some clinically relevant allergen components, such as rPru p 3 is 
      needed.
FAU - Gadisseur, Romy
AU  - Gadisseur R
AD  - Department of Clinical Chemistry, University Hospital of Liege, Belgium. 
      romy.gadisseur@chu.ulg.ac.be
FAU - Chapelle, Jean-Paul
AU  - Chapelle JP
FAU - Cavalier, Etienne
AU  - Cavalier E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20101214
PL  - Germany
TA  - Clin Chem Lab Med
JT  - Clinical chemistry and laboratory medicine
JID - 9806306
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Antibody Specificity
MH  - Female
MH  - Humans
MH  - Hypersensitivity/*diagnosis/*immunology
MH  - Immunoglobulin E/analysis/immunology
MH  - Male
MH  - Protein Array Analysis/*methods
EDAT- 2010/12/15 06:00
MHDA- 2011/05/28 06:00
CRDT- 2010/12/15 06:00
PHST- 2010/12/15 06:00 [entrez]
PHST- 2010/12/15 06:00 [pubmed]
PHST- 2011/05/28 06:00 [medline]
AID - 10.1515/CCLM.2011.052 [doi]
PST - ppublish
SO  - Clin Chem Lab Med. 2011 Feb;49(2):277-80. doi: 10.1515/CCLM.2011.052. Epub 2010 
      Dec 14.