PMID- 21143189
OWN - NLM
STAT- MEDLINE
DCOM- 20110902
LR  - 20181201
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 35
IP  - 6
DP  - 2011 Jun
TI  - Low-serum culture system improves the adipogenic ability of visceral adipose 
      tissue-derived stromal cells.
PG  - 559-68
LID - 10.1042/CBI20100406 [doi]
AB  - In obese adipose tissue, infiltrating macrophages release proinflammatory 
      cytokines that trigger insulin resistance. An adipocyte-based platform from 
      visceral fat would be useful to elucidate the pathology of adipose inflammation 
      and to develop therapeutic drugs for insulin resistance. ADSCs (adipose 
      tissue-derived mesenchymal stromal cells) expanded from subcutaneous fat are 
      intensively studied as sources for regenerative medicine. However, the adipocyte 
      culture system from visceral fat tissue has not been utilized yet. We aimed to 
      establish the bioactive adipocyte platform using ADSCs from visceral fat pad. 
      Stromal vascular fractions were processed from epididymal fat pads of 
      Sprague-Dawley rats and three human omental fat pads, and the ADSCs were expanded 
      using a low-serum culture method. The responses of ADSCs and ADSC-adipocytes 
      (their adipogenic lineages) to pioglitazone, a therapeutic drug for diabesity, 
      were evaluated by gene expression and ELISA. ADSCs (1x108) were expanded from 10 
      g of rat epididymal fat pads or human omental fat pads over five passages. Cell 
      surface marker expressions revealed that visceral ADSCs were equivalent to 
      mesenchymal stem cells. ADSC-adipocytes expanded in the low-serum culture system 
      significantly showed higher expression of adipogenic markers [PPAR (peroxisome 
      proliferator-activated receptor) gamma, LPL (lipoprotein lipase) and FABP4 (fatty 
      acid-binding protein 4)] and adipocytokines [adiponectin, resistin, leptin, PAI-1 
      (plasminogen-activator inhibitor 1) and IL (interleukin)-10] than those expanded 
      in a high-serum culture system. Pioglitazone accelerated the adipogenic induction 
      and increased adiponectin expression in human ADSCs by 57.9+/-5.8-fold 
      (mean+/-S.E.M.) relative to control cells (P<0.001). Both in rat and human 
      ADSC-adipocytes, TNF-alpha significantly induced proinflammatory cytokines [MCP-1 
      (monocyte chemoattractant protein-1) and IL-6] and suppressed adiponectin 
      expression, while pioglitazone antagonized these effects. The present findings 
      suggest that visceral ADSC-adipocytes expanded in low-serum culture would be 
      useful for adiposcience and pharmacological evaluations.
FAU - Nagasaki, Hiroshi
AU  - Nagasaki H
AD  - Department of Metabolic Medicine, Nagoya University Graduate School of Medicine, 
      Nagoya, Japan. nagasaki@med.nagoya-u.ac.jp
FAU - Shang, Qinglong
AU  - Shang Q
FAU - Suzuki, Takeshi
AU  - Suzuki T
FAU - Hashimoto, Hiroyuki
AU  - Hashimoto H
FAU - Yoshimura, Tomoko
AU  - Yoshimura T
FAU - Kondo, Taka-Aki
AU  - Kondo TA
FAU - Ozaki, Takenori
AU  - Ozaki T
FAU - Maruyama, Shouichi
AU  - Maruyama S
FAU - Jomori, Takahito
AU  - Jomori T
FAU - Oiso, Yutaka
AU  - Oiso Y
FAU - Hamada, Yoji
AU  - Hamada Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Adipokines)
RN  - 0 (Adiponectin)
RN  - 0 (Culture Media)
RN  - 0 (FABP4 protein, human)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (PPAR gamma)
RN  - 0 (Thiazolidinediones)
RN  - 130068-27-8 (Interleukin-10)
RN  - EC 3.1.1.34 (Lipoprotein Lipase)
RN  - X4OV71U42S (Pioglitazone)
SB  - IM
MH  - *Adipogenesis
MH  - Adipokines/metabolism
MH  - Adiponectin/metabolism
MH  - Animals
MH  - Culture Media/chemistry/pharmacology
MH  - Fatty Acid-Binding Proteins/metabolism
MH  - Gene Expression Regulation
MH  - Humans
MH  - Interleukin-10/metabolism
MH  - Intra-Abdominal Fat/*cytology
MH  - Lipoprotein Lipase/metabolism
MH  - PPAR gamma/metabolism
MH  - Pioglitazone
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stromal Cells/cytology/metabolism
MH  - Thiazolidinediones/pharmacology
EDAT- 2010/12/15 06:00
MHDA- 2011/09/03 06:00
CRDT- 2010/12/15 06:00
PHST- 2010/12/15 06:00 [entrez]
PHST- 2010/12/15 06:00 [pubmed]
PHST- 2011/09/03 06:00 [medline]
AID - CBI20100406 [pii]
AID - 10.1042/CBI20100406 [doi]
PST - ppublish
SO  - Cell Biol Int. 2011 Jun;35(6):559-68. doi: 10.1042/CBI20100406.