PMID- 21144504
OWN - NLM
STAT- MEDLINE
DCOM- 20110511
LR  - 20110322
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 95
IP  - 5
DP  - 2011 Apr
TI  - Two cases of sperm immotility: a mosaic of flagellar alterations related to 
      dysplasia of the fibrous sheath and abnormalities of head-neck attachment.
PG  - 1787.e19-23
LID - 10.1016/j.fertnstert.2010.11.027 [doi]
AB  - OBJECTIVE: To characterize the association of two systematic sperm defects. 
      DESIGN: Case report. SETTING: University, Interdepartmental Centre for Research 
      and Therapy of Male Infertility. PATIENT(S): Patient 1, 42 years old, and patient 
      2, 38 years old, both with severe asthenozoospermia. INTERVENTION(S): Family 
      history, physical examination, hormonal analysis, microbial assays, semen 
      analysis, transmission and scanning electron microscopy, immunocytochemistry for 
      tubulin, and fluorescence in situ hybridization (FISH) for chromosomes 18, X, and 
      Y. MAIN OUTCOME MEASURE(S): Admixture of dysplasia of the fibrous sheath (DFS) 
      and head-tail misalignment up to acephalic sperm detected by microscopic methods. 
      RESULT(S): In both patients, DFS was present in incomplete form and was 
      associated with acephalic sperm and abnormal head-tail attachment. In patient 2, 
      spermatozoa were also affected by necrosis that may cause fragmentation leading 
      to short flagella; submicroscopic examination allowed defining only the origin of 
      these "stumpy" tails. Immunofluorescence confirmed the sperm alterations. FISH 
      revealed an altered frequency of diploidy and disomy in patient 2 and a slight 
      increase in diploidy in patient 1. CONCLUSION(S): The importance of 
      ultrastructural sperm evaluation for correct identification of sperm pathologies 
      is evident, particularly regarding assisted reproduction technology and genetic 
      risk assessment.
CI  - Copyright (c) 2011 American Society for Reproductive Medicine. Published by 
      Elsevier Inc. All rights reserved.
FAU - Moretti, Elena
AU  - Moretti E
AD  - Department of Biomedical Sciences, Applied Biology Section, Interdepartmental 
      Center for Research and Therapy of Male Infertility, University of Siena, Siena, 
      Italy.
FAU - Geminiani, Michela
AU  - Geminiani M
FAU - Terzuoli, Gaia
AU  - Terzuoli G
FAU - Renieri, Tommaso
AU  - Renieri T
FAU - Pascarelli, Nicola
AU  - Pascarelli N
FAU - Collodel, Giulia
AU  - Collodel G
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20101208
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
SB  - IM
MH  - Adult
MH  - Asthenozoospermia/diagnosis/*pathology
MH  - Humans
MH  - Male
MH  - Semen Analysis
MH  - Sperm Head/*pathology/ultrastructure
MH  - Sperm Motility/physiology
MH  - Sperm Tail/*pathology/ultrastructure
MH  - Spermatozoa/*abnormalities/pathology/ultrastructure
EDAT- 2010/12/15 06:00
MHDA- 2011/05/12 06:00
CRDT- 2010/12/15 06:00
PHST- 2010/08/23 00:00 [received]
PHST- 2010/09/23 00:00 [revised]
PHST- 2010/11/09 00:00 [accepted]
PHST- 2010/12/15 06:00 [entrez]
PHST- 2010/12/15 06:00 [pubmed]
PHST- 2011/05/12 06:00 [medline]
AID - S0015-0282(10)02813-X [pii]
AID - 10.1016/j.fertnstert.2010.11.027 [doi]
PST - ppublish
SO  - Fertil Steril. 2011 Apr;95(5):1787.e19-23. doi: 10.1016/j.fertnstert.2010.11.027. 
      Epub 2010 Dec 8.