PMID- 21145044
OWN - NLM
STAT- MEDLINE
DCOM- 20110207
LR  - 20161125
IS  - 1090-2163 (Electronic)
IS  - 0008-8749 (Linking)
VI  - 267
IP  - 1
DP  - 2011
TI  - Induction of immunostimulatory cytokine genes expression in human PBMCs by a 
      novel semiquinone glucoside derivative (SQGD) isolated from a Bacillus sp. INM-1.
PG  - 67-75
LID - 10.1016/j.cellimm.2010.11.005 [doi]
AB  - In the present study, a semiquinone glucoside derivative (SQGD) isolated from a 
      radioresistant bacterium Bacillus sp. INM-1 was evaluated for its 
      immunostimulatory activities. Human peripheral blood mononuclear cells (PBMCs) 
      were stimulated by different doses (30-90 microg/ml) of SQGD for different time 
      (3-12h) intervals at 37 degrees C, and IL-12p40, IL-23p19, IL-10, RelA and c-Jun gene 
      expression analysis was carried out by qRT-PCR method. SQGD dose dependent 
      cytokines protein expression kinetic analysis was carried out using western 
      blotting. As the results of SQGD (30mug/ml) stimulation for 3h at 37 degrees C, 
      significant induction in IL-12p40, IL-23p19 and RelA gene expression was observed 
      in PBMCs compared to unstimulated control cells. However, no such induction in 
      IL-10 and c-Jun gene expression was observed. Time dependent protein expression 
      study indicated significant increase in IL-12p40, IL-12p35, IL-23p19 and RelA 
      protein expression at 3-6h, which was found decrease at 12h upon SQGD treatment. 
      In contrast, IL-10 protein expression was found to enhance significantly at 12h 
      after SQGD treatment to the PBMCs. SQGD dose dependent study showed approximately 
      similar level of induction in IL-12p40, IL-12p35, IL-23p19 and RelA proteins 
      expression at all tested concentration (30-90 microg/ml) compared to control. 
      However, no significant change in the IL-10 and c-Jun protein expression was 
      observed at any SQGD concentration. SQGD treatment (0.25mg/kgbwt.) was also found 
      to enhance anti-keyhole Limpet Hemocynin (KLH) IgM antibodies significantly in 
      the mice immunized by KLH. Thus, SQGD fraction stimulates cellular immunity by 
      inducing immunostimulatory cytokines and humoral immunity by enhancing IgM 
      antibodies and could be a promising immunostimulant. Further studies related to 
      molecular mechanisms offering immunostimulation is underway, will certainly 
      helpful to unravel its mode of action in the biological system.
CI  - 2010 Elsevier Inc. All rights reserved.
FAU - Kumar, Raj
AU  - Kumar R
AD  - Institute of Nuclear Medicine and Allied Sciences, Delhi, India. 
      rajkumar790@yahoo.com
FAU - Sharma, Rakesh Kumar
AU  - Sharma RK
FAU - Bansal, Deen Dayal
AU  - Bansal DD
FAU - Patel, Dev Dutt
AU  - Patel DD
FAU - Mishra, Saurabh
AU  - Mishra S
FAU - Miteva, Lyuba
AU  - Miteva L
FAU - Dobreva, Zlatka
AU  - Dobreva Z
FAU - Gadjeva, Veselina
AU  - Gadjeva V
FAU - Stanilova, Spaska
AU  - Stanilova S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101119
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Benzoquinones)
RN  - 0 (Cytokines)
RN  - 0 (Glucosides)
RN  - 0 (IL23A protein, human)
RN  - 0 (Interleukin-12 Subunit p35)
RN  - 0 (Interleukin-12 Subunit p40)
RN  - 0 (Interleukin-23 Subunit p19)
RN  - 0 (RELA protein, human)
RN  - 0 (Transcription Factor RelA)
RN  - 3T006GV98U (quinone)
SB  - IM
MH  - Animals
MH  - Bacillus/chemistry/*immunology
MH  - Benzoquinones/*chemistry
MH  - Cells, Cultured
MH  - Cytokines/genetics/*immunology
MH  - *Gene Expression Regulation
MH  - Glucosides/chemistry/*immunology/isolation & purification
MH  - Humans
MH  - Interleukin-12 Subunit p35/genetics/*immunology
MH  - Interleukin-12 Subunit p40/genetics/*immunology
MH  - Interleukin-23 Subunit p19/genetics/*immunology
MH  - Kinetics
MH  - Leukocytes/*immunology
MH  - Male
MH  - Mice
MH  - Transcription Factor RelA/genetics
EDAT- 2010/12/15 06:00
MHDA- 2011/02/08 06:00
CRDT- 2010/12/15 06:00
PHST- 2010/07/21 00:00 [received]
PHST- 2010/11/12 00:00 [revised]
PHST- 2010/11/15 00:00 [accepted]
PHST- 2010/12/15 06:00 [entrez]
PHST- 2010/12/15 06:00 [pubmed]
PHST- 2011/02/08 06:00 [medline]
AID - S0008-8749(10)00289-3 [pii]
AID - 10.1016/j.cellimm.2010.11.005 [doi]
PST - ppublish
SO  - Cell Immunol. 2011;267(1):67-75. doi: 10.1016/j.cellimm.2010.11.005. Epub 2010 
      Nov 19.