PMID- 21145692
OWN - NLM
STAT- MEDLINE
DCOM- 20110823
LR  - 20220408
IS  - 1873-6823 (Electronic)
IS  - 0741-8329 (Linking)
VI  - 45
IP  - 4
DP  - 2011 Jun
TI  - Effects of ethanol consumption on chromatin condensation and DNA integrity of 
      epididymal spermatozoa in rat.
PG  - 403-9
LID - 10.1016/j.alcohol.2010.10.005 [doi]
AB  - Alcohol abuse is considered as one of the problems associated with poor semen 
      production and sperm quality. Both acute and chronic alcohol consumption may 
      affect spermatozoal chromatin disorders through apoptosis. Therefore, for the 
      first time, this experimental study was performed to evaluate the effect of 
      ethanol consumption on sperm parameters and chromatin integrity of spermatozoa 
      aspirated from cauda epididymis of rats. Twenty adult Wistar rats were divided 
      into ethanol consumption and control groups. Access to ethanol and water was 
      provided ad libitum for experimental and control animals, respectively. The cauda 
      epididymal spermatozoa were aspirated for analysis of sperm parameters and sperm 
      chromatin integrity with aniline blue (AB), chromomycin A3 (CMA3), toluidine blue 
      (TB), and acridine orange (AO) assays. Sperm progressive and nonprogressive 
      motility of ethanol-consuming rats were significantly decreased compared with 
      control animals (P < .05). In addition, the rates of AB-reacted spermatozoa were 
      similar in both groups (P > .05). However, with regard to CMA3, AO, and TB 
      stainings, there was a significant increase in ethanol group when compared with 
      the controls (P < .05). The majority of TB+ and AO+ spermatozoa were higher than 
      "cut-off" value in ethanol group, whereas the mean rates of CMA3+ spermatozoa was 
      below the "cut-off" value in both groups. The results showed that ethanol 
      consumption disturbs sperm motility, nuclear maturity and DNA integrity of 
      spermatozoa in rat. Therefore, ethanol abuse results in the production of 
      spermatozoa with less condensed chromatin, and this may be one possible cause of 
      infertility following ethanol consumption.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Talebi, Ali Reza
AU  - Talebi AR
AD  - Department of Anatomy, Research and Clinical Center for Infertility, Shahid 
      Sadoughi University of Medical Sciences, Yazd, Iran. prof_talebi@ssu.ac.ir
FAU - Sarcheshmeh, Abolghasem Abbasi
AU  - Sarcheshmeh AA
FAU - Khalili, Mohammad Ali
AU  - Khalili MA
FAU - Tabibnejad, Nasim
AU  - Tabibnejad N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101210
PL  - United States
TA  - Alcohol
JT  - Alcohol (Fayetteville, N.Y.)
JID - 8502311
RN  - 0 (Chromatin)
SB  - IM
MH  - Alcoholism/complications/*physiopathology
MH  - Animals
MH  - Chromatin/*drug effects
MH  - DNA Damage/*drug effects
MH  - DNA Fragmentation
MH  - Epididymis
MH  - Infertility, Male/*chemically induced
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Sperm Motility/*drug effects
MH  - Spermatozoa/*drug effects
EDAT- 2010/12/15 06:00
MHDA- 2011/08/24 06:00
CRDT- 2010/12/15 06:00
PHST- 2009/12/13 00:00 [received]
PHST- 2010/09/30 00:00 [revised]
PHST- 2010/10/12 00:00 [accepted]
PHST- 2010/12/15 06:00 [entrez]
PHST- 2010/12/15 06:00 [pubmed]
PHST- 2011/08/24 06:00 [medline]
AID - S0741-8329(10)00173-4 [pii]
AID - 10.1016/j.alcohol.2010.10.005 [doi]
PST - ppublish
SO  - Alcohol. 2011 Jun;45(4):403-9. doi: 10.1016/j.alcohol.2010.10.005. Epub 2010 Dec 
      10.