PMID- 21148703
OWN - NLM
STAT- MEDLINE
DCOM- 20110526
LR  - 20211020
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 77
IP  - 3
DP  - 2011 Feb
TI  - Real-time PCR assay for Clostridium perfringens in broiler chickens in a 
      challenge model of necrotic enteritis.
PG  - 1135-9
LID - 10.1128/AEM.01803-10 [doi]
AB  - We compared ileal Clostridium perfringens quantification results produced by 
      real-time PCR and culture-based methods in broiler chickens in a challenge model 
      of necrotic enteritis. Assessment of the relative standard deviations (RSDs) 
      revealed that the real-time PCR assay generated a smaller standard deviation and 
      thus was more precise than the culture-based method. Linear regression analysis 
      indicated that the bacterial counts of these two methods were highly correlated 
      (R(2) = 0.845). We suggest that real-time PCR could be a replacement of the 
      culture method for quantifying C. perfringens in the intestinal tracts of broiler 
      chickens.
FAU - Wu, Shu-Biao
AU  - Wu SB
AD  - School of Environmental and Rural Science, University of New England, Armidale, 
      NSW 2351, Australia. shubiao.wu@une.edu.au
FAU - Rodgers, Nicholas
AU  - Rodgers N
FAU - Choct, Mingan
AU  - Choct M
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101210
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Culture Media)
SB  - IM
MH  - Animals
MH  - Bacterial Load
MH  - Bacteriological Techniques
MH  - Chickens/*microbiology
MH  - Clostridium perfringens/*genetics/isolation & purification
MH  - Culture Media
MH  - Enteritis/microbiology/*veterinary
MH  - Ileum/*microbiology/pathology
MH  - Intestines/microbiology/pathology
MH  - Linear Models
MH  - Necrosis/microbiology/veterinary
MH  - Polymerase Chain Reaction/*methods
MH  - Poultry Diseases/diagnosis/*microbiology
PMC - PMC3028694
EDAT- 2010/12/15 06:00
MHDA- 2011/05/27 06:00
PMCR- 2011/08/01
CRDT- 2010/12/15 06:00
PHST- 2010/12/15 06:00 [entrez]
PHST- 2010/12/15 06:00 [pubmed]
PHST- 2011/05/27 06:00 [medline]
PHST- 2011/08/01 00:00 [pmc-release]
AID - AEM.01803-10 [pii]
AID - 1803-10 [pii]
AID - 10.1128/AEM.01803-10 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2011 Feb;77(3):1135-9. doi: 10.1128/AEM.01803-10. Epub 
      2010 Dec 10.