PMID- 21148811
OWN - NLM
STAT- MEDLINE
DCOM- 20110509
LR  - 20210206
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 117
IP  - 9
DP  - 2011 Mar 3
TI  - Hypoxia modulates the gene expression profile of immunoregulatory receptors in 
      human mature dendritic cells: identification of TREM-1 as a novel hypoxic marker 
      in vitro and in vivo.
PG  - 2625-39
LID - 10.1182/blood-2010-06-292136 [doi]
AB  - Dendritic cells (DCs) are a heterogeneous group of professional 
      antigen-presenting cells functioning as sentinels of the immune system and 
      playing a key role in the initiation and amplification of innate and adaptive 
      immune responses. DC development and functions are acquired during a complex 
      differentiation and maturation process influenced by several factors present in 
      the local milieu. A common feature at pathologic sites is represented by hypoxia, 
      a condition of low pO(2), which creates a unique microenvironment affecting cell 
      phenotype and behavior. Little is known about the impact of hypoxia on the 
      generation of mature DCs (mDCs). In this study, we identified by gene expression 
      profiling a significant cluster of genes coding for immune-related cell surface 
      receptors strongly up-regulated by hypoxia in monocyte-derived mDCs and 
      characterized one of such receptors, TREM-1, as a new hypoxia-inducible gene in 
      mDCs. TREM-1 associated with DAP12 in hypoxic mDCs, and its engagement elicited 
      DAP12-linked signaling, resulting in ERK-1, Akt, and IkappaBalpha phosphorylation and 
      proinflammatory cytokine and chemokine secretion. Finally, we provided the first 
      evidence that TREM-1 is expressed on mDCs infiltrating the inflamed hypoxic 
      joints of children affected by juvenile idiopathic arthritis, representing a new 
      in vivo marker of hypoxic mDCs endowed with proinflammatory properties.
CI  - (c) 2011 by The American Society of Hematology
FAU - Bosco, Maria Carla
AU  - Bosco MC
AD  - Laboratory of Molecular Biology, G. Gaslini Institute, Genova, Italy. 
      mariacarlabosco@ospedalegaslini.ge.it
FAU - Pierobon, Daniele
AU  - Pierobon D
FAU - Blengio, Fabiola
AU  - Blengio F
FAU - Raggi, Federica
AU  - Raggi F
FAU - Vanni, Cristina
AU  - Vanni C
FAU - Gattorno, Marco
AU  - Gattorno M
FAU - Eva, Alessandra
AU  - Eva A
FAU - Novelli, Francesco
AU  - Novelli F
FAU - Cappello, Paola
AU  - Cappello P
FAU - Giovarelli, Mirella
AU  - Giovarelli M
FAU - Varesio, Luigi
AU  - Varesio L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101210
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Biomarkers)
RN  - 0 (Chemokines)
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (TREM1 protein, human)
RN  - 0 (TYROBP protein, human)
RN  - 0 (Triggering Receptor Expressed on Myeloid Cells-1)
RN  - S88TT14065 (Oxygen)
SB  - IM
CIN - Blood. 2011 Mar 3;117(9):2561-2. PMID: 21372159
MH  - Adaptor Proteins, Signal Transducing/metabolism
MH  - Arthritis, Juvenile/genetics/pathology
MH  - Biomarkers/metabolism
MH  - Cell Differentiation/drug effects/*genetics
MH  - Cell Hypoxia/drug effects/genetics
MH  - Chemokines/metabolism
MH  - Cross-Linking Reagents/pharmacology
MH  - Data Mining
MH  - Dendritic Cells/*cytology/drug effects/*metabolism
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Inflammation Mediators/metabolism
MH  - Membrane Glycoproteins/genetics/*metabolism
MH  - Membrane Proteins/metabolism
MH  - Monocytes/cytology
MH  - Oxygen/pharmacology
MH  - Phenotype
MH  - Receptors, Immunologic/*genetics/metabolism
MH  - Signal Transduction/drug effects/genetics
MH  - Synovial Fluid/drug effects/metabolism
MH  - Triggering Receptor Expressed on Myeloid Cells-1
EDAT- 2010/12/15 06:00
MHDA- 2011/05/10 06:00
CRDT- 2010/12/15 06:00
PHST- 2010/12/15 06:00 [entrez]
PHST- 2010/12/15 06:00 [pubmed]
PHST- 2011/05/10 06:00 [medline]
AID - S0006-4971(20)39059-5 [pii]
AID - 10.1182/blood-2010-06-292136 [doi]
PST - ppublish
SO  - Blood. 2011 Mar 3;117(9):2625-39. doi: 10.1182/blood-2010-06-292136. Epub 2010 
      Dec 10.