PMID- 21150162
OWN - NLM
STAT- MEDLINE
DCOM- 20110623
LR  - 20151119
IS  - 1423-002X (Electronic)
IS  - 0378-7346 (Linking)
VI  - 71
IP  - 2
DP  - 2011
TI  - Matrix metalloproteinase-3 mRNA: a promising peripheral blood marker for 
      diagnosis of endometriosis.
PG  - 118-23
LID - 10.1159/000320752 [doi]
AB  - BACKGROUND/AIMS: Endometriosis is an invasive disease. Its diagnosis depends on 
      laparoscopy, which is traumatic and associated with potential complications. The 
      aim of this study was to develop a rapid, reliable, and less invasive diagnostic 
      test for endometriosis. We hypothesized that genes related to cell invasion would 
      be transcriptionally upregulated in endometriosis, and tested whether blood 
      levels of their transcripts might be used as biomarkers of endometriosis. 
      METHODS: We used quantitative real-time reverse transcription polymerase chain 
      reaction (qRT-PCR) to quantify the mRNA levels of vascular endothelial growth 
      factor A (VEGFA), matrix metalloproteinase-3 (MMP-3), and MMP-9 in peripheral 
      blood from 20 patients with mild/intermediate endometriosis, 20 patients with 
      severe endometriosis and 20 endometriosis-free subjects. RESULTS: Our results 
      indicate that circulating mRNA for MMP-3 is significantly higher in patients with 
      endometriosis than in control patients, regardless of the degree of severity. 
      Conversely, the level of circulating mRNA for VEGFA and MMP-9 did not distinguish 
      patients from controls. CONCLUSION: MMP-3 mRNA is a promising peripheral blood 
      marker that discriminates between patients with endometriosis and healthy 
      subjects. Our results support the possibility of finding genes suitable for 
      diagnostic qRT-PCR for endometriosis in peripheral blood and should be explored 
      further.
CI  - Copyright (c) 2010 S. Karger AG, Basel.
FAU - De Sanctis, Paola
AU  - De Sanctis P
AD  - Department of Histology, Embryology and Applied Biology, University of Bologna, 
      Bologna, Italy.
FAU - Elmakky, Amira
AU  - Elmakky A
FAU - Farina, Antonio
AU  - Farina A
FAU - Caramelli, Elisabetta
AU  - Caramelli E
FAU - Seracchioli, Renato
AU  - Seracchioli R
FAU - Mabrouk, Mohamed
AU  - Mabrouk M
FAU - Mignemi, Giuseppe
AU  - Mignemi G
FAU - Venturoli, Stefano
AU  - Venturoli S
FAU - Villa, Gioia
AU  - Villa G
FAU - Guerrini, Manuela
AU  - Guerrini M
FAU - Manuzzi, Linda
AU  - Manuzzi L
FAU - Montanari, Giulia
AU  - Montanari G
FAU - Valvassori, Luisa
AU  - Valvassori L
FAU - Zucchini, Cinzia
AU  - Zucchini C
LA  - eng
PT  - Journal Article
DEP - 20101209
PL  - Switzerland
TA  - Gynecol Obstet Invest
JT  - Gynecologic and obstetric investigation
JID - 7900587
RN  - 0 (Biomarkers)
RN  - 0 (RNA, Messenger)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Adult
MH  - Biomarkers/blood
MH  - Endometriosis/blood/*diagnosis
MH  - Female
MH  - Humans
MH  - Matrix Metalloproteinase 3/*blood/genetics
MH  - Matrix Metalloproteinase 9/*blood/genetics
MH  - RNA, Messenger/*blood
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Vascular Endothelial Growth Factor A/*blood/genetics
EDAT- 2010/12/15 06:00
MHDA- 2011/06/24 06:00
CRDT- 2010/12/15 06:00
PHST- 2009/10/21 00:00 [received]
PHST- 2010/04/21 00:00 [accepted]
PHST- 2010/12/15 06:00 [entrez]
PHST- 2010/12/15 06:00 [pubmed]
PHST- 2011/06/24 06:00 [medline]
AID - 000320752 [pii]
AID - 10.1159/000320752 [doi]
PST - ppublish
SO  - Gynecol Obstet Invest. 2011;71(2):118-23. doi: 10.1159/000320752. Epub 2010 Dec 
      9.