PMID- 2115070
OWN - NLM
STAT- MEDLINE
DCOM- 19900817
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 55
IP  - 2
DP  - 1990 Aug
TI  - Neurofilament proteins are distributed in a diminishing proximodistal gradient 
      along rat sciatic nerve.
PG  - 453-60
AB  - Neurofilament (NF) proteins are distributed in a diminishing proximodistal 
      gradient along rat sciatic nerve when compared with total noncollagen or other 
      proteins in nerve. About a twofold decline of NF proteins can be detected by 
      quantitating nerve proteins that have been separated by gel electrophoresis. A 
      similar decrease of immunoreactivity to each NF subunit is seen in distal nerve 
      segments when noncollagen nerve proteins are immunoblotted. Parallel decreases 
      occur in all three NF proteins, thereby maintaining neurofilament subunit 
      stoichiometry along the neuraxis. The same NF gradient can be detected when the 
      NF contents in nerve branches to the gluteus and gastrocnemius muscles are 
      compared with each other and with those in nerve segments taken from the same 
      proximodistal levels of the parent sciatic nerve. The gradient of NF proteins 
      increases during postnatal development and is readily detected by postnatal day 
      16. During the same period of development, the heavy NF subunit appears for the 
      first time and is rapidly incorporated throughout the sciatic nerve. Hence, the 
      NF gradient becomes manifest during the development and maturation of the adult 
      form of the axonal cytoskeleton. The basis for the proximodistal gradient of NF 
      proteins in peripheral nerve is presently unknown. The extent of the gradient 
      cannot be accounted for on the basis of diminishing numbers of nerve fibers or 
      increasing amounts of other nerve proteins, e.g., collagen, in distal nerve. An 
      alternative interpretation is that the gradient reflects a low level of NF 
      protein turnover during axonal transport.
FAU - Schlaepfer, W W
AU  - Schlaepfer WW
AD  - Department of Pathology and Laboratory Medicine, University of Pennsylvania, 
      School of Medicine, Philadelphia 19104-6079.
FAU - Bruce, J
AU  - Bruce J
LA  - eng
GR  - NS 15722/NS/NINDS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Intermediate Filament Proteins)
RN  - 0 (Neurofilament Proteins)
RN  - 0 (neurofilament protein L)
RN  - 108688-71-7 (neurofilament protein H)
RN  - 111365-29-8 (neurofilament protein M)
SB  - IM
MH  - Animals
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immunoblotting
MH  - Intermediate Filament Proteins/*analysis
MH  - Molecular Weight
MH  - Nerve Fibers/analysis
MH  - Neurofilament Proteins
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Sciatic Nerve/*analysis/growth & development
MH  - Tissue Distribution
EDAT- 1990/08/01 00:00
MHDA- 1990/08/01 00:01
CRDT- 1990/08/01 00:00
PHST- 1990/08/01 00:00 [pubmed]
PHST- 1990/08/01 00:01 [medline]
PHST- 1990/08/01 00:00 [entrez]
AID - 10.1111/j.1471-4159.1990.tb04157.x [doi]
PST - ppublish
SO  - J Neurochem. 1990 Aug;55(2):453-60. doi: 10.1111/j.1471-4159.1990.tb04157.x.