PMID- 21151193
OWN - NLM
STAT- MEDLINE
DCOM- 20111206
LR  - 20180822
IS  - 1440-1711 (Electronic)
IS  - 0818-9641 (Linking)
VI  - 89
IP  - 6
DP  - 2011 Aug
TI  - Myelopoiesis related to perinatal spleen.
PG  - 689-95
LID - 10.1038/icb.2010.156 [doi]
AB  - Adult murine spleen is known to have a major role in the development of dendritic 
      cell (DC) subsets, including conventional DC and plasmacytoid DC. In this lab, 
      long-term cultures (LTCs) established from murine spleen support continuous 
      production of novel dendritic-like cells, termed LTC-DC. An in vivo equivalent 
      subset also exists in spleen, namely L-DC. As co-cultures using LTC-derived 
      splenic stroma support the outgrowth of L-DC from spleen and bone marrow sources, 
      it is likely that spleen represents an important niche for DC development. To 
      investigate the appearance of L-DC during ontogeny, spleen was isolated from 
      embryonic and neonatal mice of different ages for analysis of myeloid and DC 
      subsets. Perinatal spleen was also used to establish co-cultures for 
      identification of progenitors, and LTCs were established from spleens for 
      assessment of stromal competence. Although spleen from 16-day embryos (E16.5) 
      contained myeloid cells, DC subsets did not appear until day 4 after birth (D4). 
      However, murine spleen at D0 contained progenitors, which could seed co-cultures 
      for L-DC production. LTC could not be established from spleen until D4. The 
      appearance of L-DC after D4 in spleen is dependent on the formation of the 
      appropriate stromal microenvironment which occurs in the early postnatal period.
FAU - Hinton, Rebecca
AU  - Hinton R
AD  - Immunology and Stem Cell Laboratory, Research School of Biology, The Australian 
      National University, Canberra, Australian Capital Territory, Australia.
FAU - Petvises, Sawang
AU  - Petvises S
FAU - O'Neill, Helen
AU  - O'Neill H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101214
PL  - United States
TA  - Immunol Cell Biol
JT  - Immunology and cell biology
JID - 8706300
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/immunology/metabolism
MH  - Hematopoietic Stem Cells/cytology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Myeloid Cells/immunology/metabolism
MH  - Myelopoiesis/*immunology
MH  - Spleen/embryology/growth & development/*immunology
EDAT- 2010/12/15 06:00
MHDA- 2011/12/13 00:00
CRDT- 2010/12/15 06:00
PHST- 2010/12/15 06:00 [entrez]
PHST- 2010/12/15 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - icb2010156 [pii]
AID - 10.1038/icb.2010.156 [doi]
PST - ppublish
SO  - Immunol Cell Biol. 2011 Aug;89(6):689-95. doi: 10.1038/icb.2010.156. Epub 2010 
      Dec 14.