PMID- 21152936
OWN - NLM
STAT- MEDLINE
DCOM- 20110624
LR  - 20110202
IS  - 1432-0878 (Electronic)
IS  - 0302-766X (Linking)
VI  - 343
IP  - 2
DP  - 2011 Feb
TI  - Enhanced expression of fibroblast growth factor 2 in bone marrow cells and its 
      potential role in the differentiation of hepatic epithelial stem-like cells into 
      the hepatocyte lineage.
PG  - 371-8
LID - 10.1007/s00441-010-1093-2 [doi]
AB  - The transplantation of bone marrow cells (BMCs) has been applied in liver 
      regenerative cell therapy. However, details of the interaction between the 
      transplanted BMCs and hepatic stem cells have not been elucidated. The aim of the 
      present study was to investigate the interaction of BMCs with hepatic stem-like 
      cells (HSLCs) and to determine the BMC factor that steers HSLC differentiation 
      into the hepatocyte lineage. Both BMCs and HSLCs were obtained from an adult 
      Sprague-Dawley rat, and a co-culture system was established. Cell proliferation 
      was analyzed by a proliferation assay, and the differentiation of HSLCs into the 
      hepatocyte lineage was evaluated by the detection of cellular mRNA for 
      liver-specific proteins. DNA microarray analysis was applied to BMCs co-cultured 
      with HSLCs to determine the genes upregulated by their interaction. The 
      proliferation of HSLCs co-cultured with BMCs was significantly higher than that 
      of HSLCs cultured alone, and the expression of mRNAs for both albumin and 
      tryptophan-2,3-dioxygenase was detectable in the co-cultured HSLCs. DNA 
      microarray analysis showed the upregulated expression of fibroblast growth factor 
      2 (FGF2) mRNA in BMCs co-cultured with HSLCs, and the expression of mRNAs for 
      both albumin and tyrosine aminotransferase became detectable in HSLCs cultured 
      with FGF2. Thus, BMCs stimulate both the proliferation of HSLCs and their 
      differentiation into the hepatocyte lineage. FGF2 is one of the factors that is 
      produced by the interacting BMCs and that stimulates this differentiation.
FAU - Haga, Hiroaki
AU  - Haga H
AD  - Department of Gastroenterology, Yamagata University School of Medicine, 2-2-2 
      Iida-nishi, Yamagata, 990-9585, Japan.
FAU - Saito, Takafumi
AU  - Saito T
FAU - Okumoto, Kazuo
AU  - Okumoto K
FAU - Ugajin, Satoshi
AU  - Ugajin S
FAU - Sato, Chikako
AU  - Sato C
FAU - Ishii, Rika
AU  - Ishii R
FAU - Nishise, Yuko
AU  - Nishise Y
FAU - Ito, Junitsu
AU  - Ito J
FAU - Watanabe, Hisayoshi
AU  - Watanabe H
FAU - Saito, Koji
AU  - Saito K
FAU - Togashi, Hitoshi
AU  - Togashi H
FAU - Kawata, Sumio
AU  - Kawata S
LA  - eng
PT  - Journal Article
DEP - 20101209
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
RN  - 0 (RNA, Messenger)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - *Cell Differentiation
MH  - *Cell Lineage
MH  - Cell Proliferation
MH  - Epithelial Cells/cytology/*metabolism
MH  - Fibroblast Growth Factor 2/genetics/*metabolism
MH  - Hepatocytes/*cytology/metabolism
MH  - Male
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stem Cells/*cytology/metabolism
EDAT- 2010/12/15 06:00
MHDA- 2011/06/28 06:00
CRDT- 2010/12/15 06:00
PHST- 2010/08/04 00:00 [received]
PHST- 2010/11/17 00:00 [accepted]
PHST- 2010/12/15 06:00 [entrez]
PHST- 2010/12/15 06:00 [pubmed]
PHST- 2011/06/28 06:00 [medline]
AID - 10.1007/s00441-010-1093-2 [doi]
PST - ppublish
SO  - Cell Tissue Res. 2011 Feb;343(2):371-8. doi: 10.1007/s00441-010-1093-2. Epub 2010 
      Dec 9.