PMID- 21153730
OWN - NLM
STAT- MEDLINE
DCOM- 20110718
LR  - 20211020
IS  - 1432-0991 (Electronic)
IS  - 0343-8651 (Linking)
VI  - 62
IP  - 4
DP  - 2011 Apr
TI  - Identification of two species of yeast-like symbiotes in the brown planthopper, 
      Nilaparvata lugens.
PG  - 1133-8
LID - 10.1007/s00284-010-9830-z [doi]
AB  - To determine the species of the yeast-like symbionts (YLS) in the brown 
      planthoppers (BPH), Nilaparvata lugens, YLS were first isolated and purified by 
      ultracentrifugation from the fat bodies of BPH, and then 18S rDNA and internal 
      transcribed spacer (ITS)-5.8S rDNA sequences of YLS were amplified with the 
      different general primers for fungi. The results showed that the two different 
      18S and ITS-5.8S rDNA sequences of YLS were obtained. One 2291-bp DNA sequence, 
      which contained 18S and ITS-5.8S rDNA, showed the high similarity to Cryptococcus 
      and was named Cryp-Like symbiotes. Another 1248-bp DNA sequence, which contained 
      a part of 18S and ITS-5.8S rDNA, showed the high similarity to Pichia 
      guilliermondii and was named Pichia-Like symbiotes. It was further proved that 
      Cryp- and Pichia-Like symbiotes existed in BPH through nested PCR with specific 
      primers for two symbiotes and in situ hybridization analysis using 
      digoxigenin-labeled probes. Our results showed that BPH harbored more than one 
      species of eukaryotic YLS, which suggested that diversity of fungal endosymbiotes 
      may be occurred in planthoppers, just like bacterial endosymbiotes.
FAU - Dong, ShengZhang
AU  - Dong S
AD  - Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, 
      China Jiliang University, Hangzhou, Zhejiang 310018, China. dong_shzhang@163.com
FAU - Pang, Kun
AU  - Pang K
FAU - Bai, Xu
AU  - Bai X
FAU - Yu, XiaoPing
AU  - Yu X
FAU - Hao, PeiYing
AU  - Hao P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101214
PL  - United States
TA  - Curr Microbiol
JT  - Current microbiology
JID - 7808448
RN  - 0 (DNA, Fungal)
RN  - 0 (RNA, Ribosomal, 18S)
RN  - 0 (RNA, Ribosomal, 5.8S)
SB  - IM
MH  - Animals
MH  - Cryptococcus/classification/genetics/*isolation & purification/physiology
MH  - DNA, Fungal/genetics
MH  - Hemiptera/*microbiology/physiology
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Pichia/classification/genetics/*isolation & purification/physiology
MH  - RNA, Ribosomal, 18S/genetics
MH  - RNA, Ribosomal, 5.8S/genetics
MH  - *Symbiosis
EDAT- 2010/12/15 06:00
MHDA- 2011/07/19 06:00
CRDT- 2010/12/15 06:00
PHST- 2010/07/02 00:00 [received]
PHST- 2010/11/16 00:00 [accepted]
PHST- 2010/12/15 06:00 [entrez]
PHST- 2010/12/15 06:00 [pubmed]
PHST- 2011/07/19 06:00 [medline]
AID - 10.1007/s00284-010-9830-z [doi]
PST - ppublish
SO  - Curr Microbiol. 2011 Apr;62(4):1133-8. doi: 10.1007/s00284-010-9830-z. Epub 2010 
      Dec 14.