PMID- 21153865
OWN - NLM
STAT- MEDLINE
DCOM- 20110715
LR  - 20211020
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 350
IP  - 1-2
DP  - 2011 Apr
TI  - The role of glucagon-like peptide-2 on apoptosis, cell proliferation, and 
      oxidant-antioxidant system at a mouse model of intestinal injury induced by tumor 
      necrosis factor-alpha/actinomycin D.
PG  - 13-27
LID - 10.1007/s11010-010-0678-0 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a multifunctional cytokine, which has the 
      ability to produce cytotoxicity via induction of cell death and cell cycle 
      arrest. Blocking the synthesis of protective proteins through a transcriptional 
      inhibitor such as actinomycin D (Act D) sensitizes many cell types to TNF-alpha 
      toxicity. Teduglutide, h[Gly(2)]GLP-2, is a protease-resistant synthetic analog 
      of glucagon-like peptide-2 (GLP-2) which is an intestinotrophic peptide. In this 
      study, we evaluated this potential of GLP-2 on apoptosis, cell proliferation, and 
      oxidant-antioxidant system on a mouse model of intestinal injury induced by 
      TNF-alpha/Act D. The intestinal injury was induced by intraperitoneal administration 
      of 15 mug/kg TNF-alpha and 800 mug/kg Act D per mouse. Animals were injected 
      subcutaneously 200 mug/kg h[Gly(2)]GLP-2 every 12 h for 10 consecutive days prior 
      to the administration of TNF-alpha and Act D. The model of intestinal injury induced 
      by TNF-alpha/Act D, which is the new animal model for the intestinal disorders, was 
      characterized by the degeneration of intestinal mucosa, an increase in apoptotic 
      index, expression of active caspase-3, lipid peroxidation and glutathione (GSH) 
      levels, glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities; a 
      decrease in cell proliferation and catalase (CAT) activity. h[Gly(2)]GLP-2 
      pretreatment prevented the TNF-alpha/Act D-induced oxidative injury by a significant 
      reduction in the intestinal injury, apoptotic index, expression of active 
      caspase-3, lipid peroxidation and GSH levels, GPx and SOD activities; a markedly 
      increase in cell proliferation, and CAT activity. These results demonstrate that 
      GLP-2 has a protective, antiapoptotic, proliferative, and antioxidant effects 
      against to TNF-alpha/Act D-induced intestinal injury. It is suggested that GLP-2 may 
      potentially be useful as a therapeutic agent in TNF-alpha-mediated intestinal 
      disorders.
FAU - Arda-Pirincci, Pelin
AU  - Arda-Pirincci P
AD  - Department of Biology, Istanbul University, Istanbul, Turkey. 
      parda@istanbul.edu.tr
FAU - Bolkent, Sehnaz
AU  - Bolkent S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101214
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Antioxidants)
RN  - 0 (Gastrointestinal Agents)
RN  - 0 (Glucagon-Like Peptide 2)
RN  - 0 (Oxidants)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 1CC1JFE158 (Dactinomycin)
SB  - IM
MH  - Animals
MH  - Antioxidants/*metabolism
MH  - *Apoptosis/drug effects/physiology
MH  - *Cell Proliferation/drug effects
MH  - Dactinomycin
MH  - Disease Models, Animal
MH  - Drug Evaluation, Preclinical
MH  - Gastrointestinal Agents/pharmacology/therapeutic use
MH  - Glucagon-Like Peptide 2/pharmacology/*physiology/therapeutic use
MH  - Intestinal Diseases/chemically induced/drug therapy/metabolism/*pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Oxidants/*metabolism
MH  - Tumor Necrosis Factor-alpha
EDAT- 2010/12/15 06:00
MHDA- 2011/07/16 06:00
CRDT- 2010/12/15 06:00
PHST- 2010/06/28 00:00 [received]
PHST- 2010/12/02 00:00 [accepted]
PHST- 2010/12/15 06:00 [entrez]
PHST- 2010/12/15 06:00 [pubmed]
PHST- 2011/07/16 06:00 [medline]
AID - 10.1007/s11010-010-0678-0 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2011 Apr;350(1-2):13-27. doi: 10.1007/s11010-010-0678-0. Epub 
      2010 Dec 14.