PMID- 21154323
OWN - NLM
STAT- MEDLINE
DCOM- 20110311
LR  - 20111117
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 27
IP  - 6
DP  - 2010 Dec
TI  - [Establishment of allele specific primer polymerase chain reaction sequence-based 
      typing and investigation of the polymorphism in exon 3 of HLA-DRB1].
PG  - 639-43
LID - 10.3760/cma.j.issn.1003-9406.2010.06.008 [doi]
AB  - OBJECTIVE: To establish the allele specific primer polymerase chain reaction 
      sequence-based typing (PCR-SBT) and investigate the polymorphism of exon 3 of 
      human leukocyte antigen( HLA)-DRB1. METHODS: The fragment containing exons 2 and 
      3 of HLA-DRB1 gene was amplified by group specific primers. The amplified 
      products were digested by restriction enzymes and directly sequenced in both 
      directions. The genotype was assigned by using Assign 3.5 SBT software. RESULTS: 
      The exon 3 sequences of HLA-DRB1*08:09 and HLA-DRB1*12:02:01 were identified for 
      the first time. There were 27 polymorphic sites in exon 3 among the twenty-five 
      HLA-DRB1 alleles, which was 9.56% of all nucleotides of exon 3. The method could 
      discriminate the HLA-DRB1*14:01:01/14:54 ambiguous samples, and the 
      HLA-DRB1*14:01:01 was identified in the Chinese population. CONCLUSION: The 
      PCR-SBT method for exon 3 of HLA-DRB1 from the present study was reliable and 
      there were polymorphisms in exon 3 of HLA-DRB1.
FAU - Zhu, Fa-ming
AU  - Zhu FM
AD  - Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of 
      Ministry of Health of China, Hangzhou, Zhejiang, P.R. China. zfm00@hotmail.com
FAU - He, Yan-min
AU  - He YM
FAU - Tao, Su-dan
AU  - Tao SD
FAU - Zhang, Wei
AU  - Zhang W
FAU - Wang, Wei
AU  - Wang W
FAU - He, Jun-jun
AU  - He JJ
FAU - Lv, Hang-jun
AU  - Lv HJ
FAU - Yan, Li-xing
AU  - Yan LX
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (DNA Primers)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB1 Chains)
SB  - IM
MH  - *Alleles
MH  - Base Sequence
MH  - DNA Primers/*genetics
MH  - Evolution, Molecular
MH  - Exons/*genetics
MH  - Genotype
MH  - HLA-DR Antigens/*genetics
MH  - HLA-DRB1 Chains
MH  - Humans
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Polymerase Chain Reaction/*methods
MH  - Polymorphism, Genetic/*genetics
EDAT- 2010/12/15 06:00
MHDA- 2011/03/12 06:00
CRDT- 2010/12/15 06:00
PHST- 2010/12/15 06:00 [entrez]
PHST- 2010/12/15 06:00 [pubmed]
PHST- 2011/03/12 06:00 [medline]
AID - 940627126 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2010.06.008 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2010 Dec;27(6):639-43. doi: 
      10.3760/cma.j.issn.1003-9406.2010.06.008.